Academic journal article Iranian Journal of Public Health

Physicochemical Methods in Endotoxin Removal from Recombinant Active Pharmaceutical Ingredient (Api)

Academic journal article Iranian Journal of Public Health

Physicochemical Methods in Endotoxin Removal from Recombinant Active Pharmaceutical Ingredient (Api)

Article excerpt

Background: In this research, physical and chemical meth- ods were used and the efficiency of each of those evaluated.

Methods: In this research we have selected ultrafiltration (UF) 10KD as a physical method and incubation with sodi- um deoxicolate as a chemical method.The above methods are some example of effective processes in endotoxin removal. TAGUCHI experimental designe software was used by 2 factors: time and sodium deoxicolate concentration were examined at three levels. Time was examined at three levels of 0, 12, and 24 h and sodium deoxicolate Concentration at three levels 0.5%, 1% and 1.5%.

Results: By using of TAGUCHI designing method, 9 exper- iments were designed. En dotoxin levels were measured by a Limulus Amebocyte Lysate gel-clot assay and showed that incubation with 1. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.