Academic journal article Iranian Journal of Public Health

Overproduction of Doxorubicin and Daunorubicin in Streptomyces Peucetius by Pcr Targeting

Academic journal article Iranian Journal of Public Health

Overproduction of Doxorubicin and Daunorubicin in Streptomyces Peucetius by Pcr Targeting

Article excerpt

Background: Streptomyces peucetius, a microorganism that pro- duces the anticancer drugs do xorubicin and daunorubicin, is itself resistant to the action of these drugs. Biosynthesis of doxorubicin (DXR) is tightly regulated, limiting its produc- tion in S. peucetius cultures. The genes conferring resistance to doxorubicin and daunorubicin in S. peucetius have been sequenced. Two open reading frames, drrA and drrB, were proposed to encode for an ABC (ATP-binding cassette) type of permease that carries out export of the antibiotics in an ATP-dependent manner.

Methods: This article reports a simplified variation of PCR targeting to introduce point mutations into promoter of the drrA and drrB genes of S. peucetius ATCC 27952. The re- sistance genes drrAB from S. peucetius were cloned into the pIBR25 expression vector under a strong ermE* promoter to enhance doxorubicin (DXR) production. …

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