Academic journal article Iranian Journal of Public Health

Three Dimentional Structure Prediction of Chimeric Protein Comprising Fimh and Flic

Academic journal article Iranian Journal of Public Health

Three Dimentional Structure Prediction of Chimeric Protein Comprising Fimh and Flic

Article excerpt

Background: In this in silico study 3D structure of Chimeric protein including FimH and FliC, an appropriate vaccine candidate, is predicted.

Methods: The Protein sequence of FimH and FliC were collected from UniProt. These proteins were fused together by (EAAAK)4 linker. The secondary structure of hypothet- ical protein was predicted by GORIV secondary structure prediction method. For 3D structure prediction, I-TASSER ab initio online software,was used. The predicted structure was validated by Ramachandran plot in PROCHECK soft- ware.

Results: The top three structures were selected and refined with kobamin software that reduces the structures errors. The refined structures were evaluated with Qmean score and the quality of structures were observed to have improved.The Ramachandran plot analysis revealed that 73% of amino acid residues were incorporated in the favored regions. …

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