Academic journal article Iranian Journal of Public Health

Protection against P. Aeruginosa with a Synthetic Peptide Derived from Omp F Conjugated to Outer Membrane Vesicle (Omv) of Neisseria Meningitidis

Academic journal article Iranian Journal of Public Health

Protection against P. Aeruginosa with a Synthetic Peptide Derived from Omp F Conjugated to Outer Membrane Vesicle (Omv) of Neisseria Meningitidis

Article excerpt

Background: Pseudomonas aeruginosa is an opportunistic path- ogen that causes serious infections, is usually resistant to an- timicrobial agents and is the leading cause of morbidity and premature mortality in hospitalized and burned patients.

Methods: In this work, the bioinformatics approach was used to design and synthesis a peptide derived from OMPF (ERRANAVRDVLVNEY).This peptide contained both B- and T-cell epitopes based on prediction models. We conju- gated this peptide to the outer membrane vesicle (OMV) of Neisseria meningitidis serogroup B, which is a safe carrier protein, and evaluated its efficacy in mice.

Results: Results of this study on mice showed that the con- jugate elicited anti-alginate-IgG that were not detected after immunization with naive peptide. IgG1 was also dominant among IgG subclasses. Immunization of mice with the pep- tide-OMV conjugate raised the levels of opsonic antibodies, and the vaccinated mice were protected when challenged intranasally with P. …

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