Academic journal article Iranian Journal of Public Health

Elimination of Bacterial and Fungal Contaminations from Microalgal Culture

Academic journal article Iranian Journal of Public Health

Elimination of Bacterial and Fungal Contaminations from Microalgal Culture

Article excerpt

Background: The use of antibiotics is one of the means of dealing with contamination, but it should be noted that Chlo- rella is eukaryotic and anti-fungal compound might have neg- ative effects on it.

Methods: The alga strain used in this experiment was Chlo- rella volgaris which was isolated from fresh water.It was pure but contaminated by bacteria and fungi. 1.5% Microbiologi- cal agar (Merck, Germany) was added to modified Bristol medium to prepare a solid medium. To eliminate bacterial contamination, the filtered-pharmaceutical tetracycline, ampi- cillin, and penicillin G have been used at 0.01 g/ml concen- tration in the medium. Pharmaceutical nystatin in 40 to 100 µg / ml Concentrations has been used and added to the me- dium in petri dishes in order to eliminate the fungal contami- nation. The control culture did not have any antibiotics.This experiment carried out in three repetitions. Cultures were incubated at room temperature under a 4000 lux light intensi- ty with a 14: 10 h light photoperiod for 10 days in order to obtain free bacterial and fungal Chlorella colonies. …

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