Academic journal article Iranian Journal of Public Health

Secretory Expression of Hemagglutinin Globular Domain (Ha1) of the Influenza a (H5n1) Virus in Bacillus Subtilis

Academic journal article Iranian Journal of Public Health

Secretory Expression of Hemagglutinin Globular Domain (Ha1) of the Influenza a (H5n1) Virus in Bacillus Subtilis

Article excerpt

Background: Here we produced secretory recombinant HA1 protein in B. subtilis.

Methods: The PCR-amplified HA1 sequence was cloned into pGEM® 5Zf(-)vector and subjected for sequencing. It was then subcloned into E.coli- B.subtilis shuttle vector PHT43 and transferred to E.coli for replication. The recombinant plasmid PHT43-HA1 was extracted from E.coli and used to transform of B.subtilis by electroporation. Following the veri- fication of the new recombinant B.subtilis, it was subjected for HA1 production by IPTG induction. Total cell protein and the protein secreted into media were analysed through a time course using SDS-PAGE.

Results: The accuracy of PHT43-HA1 construct was con- firmed by Sequencing and enzymatic digestion analysis. SDS- PAGE results showed that the recombinant HA1 protein was successfully expressed and secreted into medium. …

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