Academic journal article Iranian Journal of Public Health

Taken the Soluble Mutant Form of Rice-Nsltp2 in E.coli, Strategies and Challenges

Academic journal article Iranian Journal of Public Health

Taken the Soluble Mutant Form of Rice-Nsltp2 in E.coli, Strategies and Challenges

Article excerpt

Background: In this paper, a soluble form of mutant type from Rice-nsLTP2 is expressed using fusion protein technol- ogy.

Methods: The designed mutant construct was subjected to expression and optimization in E.coli . E.coli BL21 (DE3) pLysS was the host bacterial strain for the pET32a vector expression system. The protein expression was optimized by changing in temperature, time and IPTG concentration. The production of mutant nsLTP2 was analyzed by SDS-PAGE.

Results: The new construct was transformed by heat shock procedure and the soluble recombinant nsLTP2 was ex- pressed. Therefore, the fusion protein technology was men- tioned to overcome difficulties in protein solubility. The new construct has thioredoxin tag that improves the yield of ac- tive soluble protein.

Conclusion: In the present study, we have obtained manipu- lated ns-LTPs. …

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