Academic journal article Iranian Journal of Public Health

Determining the Epidemiological Correlation of Coagulase Positive Staphylococci Isolated from Sweetmeats and Staff by Pcr- Rflp-Based Method

Academic journal article Iranian Journal of Public Health

Determining the Epidemiological Correlation of Coagulase Positive Staphylococci Isolated from Sweetmeats and Staff by Pcr- Rflp-Based Method

Article excerpt

Background: Microbial confectionary contamination in the world is widespread. Confectionary products form an im- portant part of foodstuffs in this country. The aim of this study is to isolate the epidemiological correlation of coagulase positive staphylococci isolated from sweetmeats and staff by PCR- RFLP-based method.

Methods: We randomly selected 300 samples in some con- fectionary workshops in Khoramabad city and tested them for possible contamination using the related standards of Organization of Standards and Industrial Research. We also gathered 91 swabs of the nose fluid samples of the staff. Considering the existence of coagulase gene in the samples, we used a PCR- RFLP test to identify Staphylococcus aureus. We also used AluI digesting enzyme to determine the epidemio- logical correlation of the isolates. We used IBM SPSS Statis- tics 19 to analyze the data.

Results: By PCR amplification of coagulase gene, S. aureus was detected in 21 fragments (7%) of the sweetmeat samples. Furthermore, 13 fragments (14. …

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