Academic journal article Iranian Journal of Public Health

Study of Microsporidiosis in Corneal Scraping of Keratitis Patients

Academic journal article Iranian Journal of Public Health

Study of Microsporidiosis in Corneal Scraping of Keratitis Patients

Article excerpt

Background: The current study aimed to investigate the microsporidial keratitis in patients referred to the corneal clinic of Farabi hospital, Iran, and compare with other coun- tries.

Methods: Two scraping samples from each 91 keratitis pa- tient referred to the Farabi hospital were collected by an oph- thalmologist. In order to culture, the microorganisms in a Vero cell culture, one sample was stored in microtubes con- taining PBS while the other sample used for the preparation of Giemsa and/or Gram staining slides. The samples cul- tured in Vero cell culture, and after 30 days, the cells scrapped, centrifuged and used for DNA extraction and the Nested PCR-based detection method. The Nested primer pairs designed by Using CLC Genomics workbench V.3.6.1 software to amplify all major microsporidian pathogens re- ported to infect cornea; such as E. cuniculi, E. hellem, E. intestinalis and Vittaforma corneae, as well as E. bieneusi that isolated from stool to use as positive control.

Results: Results from the nested PCR showed a negative presence of microsporidium in all the samples and the Gram and Giemsa staining also all were negative in the detection of any spores. …

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