Academic journal article Iranian Journal of Public Health

PREVALENCE OF STAPHYLOCOCCUS SPP. AND MECA GENE FROM SPECIMENS IN KURDISTAN MEDICAL UNIVERSITY HOSPITALS, Iran

Academic journal article Iranian Journal of Public Health

PREVALENCE OF STAPHYLOCOCCUS SPP. AND MECA GENE FROM SPECIMENS IN KURDISTAN MEDICAL UNIVERSITY HOSPITALS, Iran

Article excerpt

Background: Staphylococcus aureus is a major human patho- gen. Methicillin-resistant S. aureus (MRSA) have greater viru- lence than methicillin-susceptible strains. MRSA is a major cause of nosocomial and community infections,The aim of this study was a survey on prevalence of isolated MRSA from nosocomial infections and environmental specimens in Kur- distan University Hospitals.

Methods: In this study, 264 samples were collected. Samples confirmed with biochemical tests for S. aureus. 156 staphylo- cocci bacteria were related to clinical samples, 46 environ- mental samples and 59 samples were related to staff in hospi- tals war ds. Sensitivity to antibiotics was determined by using Kirby-Bauer methods with gentamicin (10µg), vancomycin (30µg), ciprofloxacin (5µg), and erythromycin (15µg). Also agar screening with 6 µg/ml oxacillin and PCR were per- formed for predicting mecA gene

Results: Frequency of isolated Staphylococcus was follow- ing: In staff, S. aureus was18(36.73), S. epidermidis was 31 (63.27%) and S. saprophyticus was not found. In environ- mental samples, S. aureus 20 (33.9%), S. epidermidis 38 (64.41%) and S. saprophyticus 1 (1.69%) were found. In Clinical samples, S. aureus was 50 (32.05%), S. epidermidis was 101 (64.74%) and S. saprophyticus was 5 (3.21%). Results showed that vancomycin (69. …

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