Academic journal article Iranian Journal of Public Health

Molecular Typing of Methicillin-Resistant Staphylococcus Aureus Strains by Pcr-Rflp of Spa Gene

Academic journal article Iranian Journal of Public Health

Molecular Typing of Methicillin-Resistant Staphylococcus Aureus Strains by Pcr-Rflp of Spa Gene

Article excerpt

Background: Protein A of Staphylococcus aureus is a pathogen- ic factor whose encoding gene, spa, shows a variation in length in different strains.In this study to characterize methi- cillin-resistant Staphylococcus aureus (MRSA) strains we applied molecular typing based on polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) of spa gene.

Methods: Studies were undertaken on 116 MRSA isolates of Staphylococcus aureus which we were isolated from Qazvin and Tehran hospitals, Iran. Methicillin resistance first determined by disc diffusion and agar screen tests and then confirmed by PCR using specific primers of mecA gene. Isolates were typed by PCR-RFLP of spa gene using Hin6I restriction en- zyme. Analysis of the amplified spa gene fragment of the representative RFLP patterns was performed with standard protocols.

Results: All the isolates resistant to methicillin were found to contain mecA gene. …

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