Academic journal article Iranian Journal of Public Health

Eric Fingerprinting and Genotyping of E. Coli Strains Isolated from 5 Surface Water Sources of Tehran

Academic journal article Iranian Journal of Public Health

Eric Fingerprinting and Genotyping of E. Coli Strains Isolated from 5 Surface Water Sources of Tehran

Article excerpt

Background: The aim of the current study was to apply ERIC-PCR method for molecular typing of E. coli strains isolated from different surface water sources in Tehran prov- ince.

Methods: Current study included E. coli strains all isolated from 5 different surface water sources of Tehran province in 2013 including Bilaghan, kan, sohanak, Jalalieh and 4&5. Bac- terial isolates were all detected and identified by standard microbiological and biochemical tests. Genomic DNA was extracted applying AccuPrep® Genomic DNA Extraction Kit and genomic fingerprints and genetic relationship be- tween the strains was evaluated by ERIC-PCR using specific primers. The PCR amplicons were visualized after electro- phoresis and staining with SYBR green and dendogram was constructed based on Dice Comparison method and UP- GMA Clustering method applying online in silico tool.

Results: One hundred and six E. coli isolates were collected from Tehran surface water sources. …

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