Academic journal article Iranian Journal of Public Health

Different Phenotypic Aspects with No Genotypic Heterogeneity in Leishmania Major Isolates of Suspected Patients in Northern Khuzestan Province

Academic journal article Iranian Journal of Public Health

Different Phenotypic Aspects with No Genotypic Heterogeneity in Leishmania Major Isolates of Suspected Patients in Northern Khuzestan Province

Article excerpt

Background: In this inquiry, behavioral aspects of L.major isolates are evaluated in two forms of genotypic and phenotypic characters among suspected patients of some exceptional zoonotic cutaneous leishmaniasis (ZCL) locations in northern Khuzestan, Iran.

Methods: A total, 200 suspected patients were collected in ZCL foci over two successive years in 2012-14. Prepared slides were stained, scaled and measured precisely by ocular micrometer and Dino-captureu under a light microscope. Whole DNA was extracted directly from smears; ITS-rDNA gene amplified, subsequently amplicons digested with HaeIII restriction enzyme. In order to discriminating of Leishmania species, PCR-RFLP, sequencing and phylogenetic analyses were established by CLC DNA Workbench 5.05, Sequencher Tmv 4.0.4 and PAUP software respectively.

Results: Only L. major was identified in suspected patients with different regular (oval or round) and irregular (spindle, pear or cigarette) morphometric amastigotes' shapes which had the average size of 3-4.5 m into each of the dry and mixed, classical and non-classical wet lesions, All sequenced ITS-rDNA gene of L. major (27 sequences) did not have any variation (? …

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