Academic journal article Iranian Journal of Public Health

EVALUATION OF THE GENETIC SIMILARITY OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED FROM IranIAN AND AFGHAN TB PATIENTS USING RFLP-BASED METHODS

Academic journal article Iranian Journal of Public Health

EVALUATION OF THE GENETIC SIMILARITY OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED FROM IranIAN AND AFGHAN TB PATIENTS USING RFLP-BASED METHODS

Article excerpt

Background: Tuberculosis is an old problem that is current- ly considered a great challenge. Noticing Iran's borders with Afghanistan and Pakistan, which are among the 22 high bur- den countries around the world. this study awareness of pos- sible genetic similarities between Mycobacterium tuberculosis iso- lated from Iranian and Afghan patient living in Markazi prov- ince, also examines the role of migration based on the epi- demiology of tuberculosis using RFLP (Restriction fragment length polymorphism) was performed.

Methods: In this study, 57 sputum specimens from smear positive patients admitted to health centers in Markazi prov- ince were cultured on specific mycobacterial culture media. Genomic DNA was extracted by standard protocols of WHO and digested separately by PvuII and AluI. Electro- phoresis was performed and DNA fragments were trans- ferred to positively charged nylon membrane by southern blotting method and hybridization by PGRS, DR and IS6110 probes. The hybridized strains were subsequently detected by enzymatic reaction and analyzed by Gel pro analyzer soft- ware.

Results: In this study a sample of mycobacteria isolated from Afghan patients were similar to isolates from Iranian patients and this similarity using all methods used in the study was confirmed

Conclusion: The results of this research based on genetic similarity, suggesting activation of circulating Mycobacterium tuberculosis strains among Iranian and Afghan nationals. …

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