Academic journal article Iranian Journal of Public Health

Effect of Long-Term Exposure of Candida Albicans to Mobile Phone Radiation on Its Aint1 Gene Sequence

Academic journal article Iranian Journal of Public Health

Effect of Long-Term Exposure of Candida Albicans to Mobile Phone Radiation on Its Aint1 Gene Sequence

Article excerpt

Background: In this study, for the first time, the mutagenic impact of long-term exposure to 900-MHz radiation on Can- dida albicans alpha-Int1 gene sequences were evaluated.

Methods: A standard EMF generator with output ranging from 860 to 940 MHz was used for radiation. 10 ml volumes from a stock suspension of Candida albicans (2x10^6 cell/ml, in log phase of growth, prepared in PBS) were transferred into ten 15 ml volume polystyrene tubes. 5 tubes were incu- bated at 4°C and exposed (distance=0~15 cm) to fixed mag- nitude of radiation with different time periods of 10, 70, 210,350 and 490 hours. The other 5 tubes were kept at the same conditions but far enough from radiation generator. Exposed and unexposed samples went under genomic DNA extraction using a standard method. PCR amplification of aInt1 gene sequence was done using a set of primers (for- ward and reverse) with optimized conditions. PCR products were resolved using 1.5% agarose gel electrophoresis and the nucleotide sequences were determined using Sanger method.

Results: All exposed and unexposed samples showed a clear electrophoretic band around 441 bp and further sequencing of the products revealed the amplified DNA segments are exactly related to alpha-Int1 gene of Candida albicans . …

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