Academic journal article Iranian Journal of Public Health

A Survey on the Antimicrobial Effect of Methanolic Extract and Alkaloid Fractions of Aerial Parts of Glaucium Vitellinum against Different Bacterial Strains and Candida Spp with Disk Diffusion and Mic

Academic journal article Iranian Journal of Public Health

A Survey on the Antimicrobial Effect of Methanolic Extract and Alkaloid Fractions of Aerial Parts of Glaucium Vitellinum against Different Bacterial Strains and Candida Spp with Disk Diffusion and Mic

Article excerpt

Background: In this study the antibacterial effect of Glauci- um vitellinum against some bacterial strains and C. albicans were evaluated by disk diffusion and microdilution methods.

Methods: Aerial parts of the plant were collected in April- May 2012 from Khansar and Golpayegan heights, Isfahan province, Iran and dried by natural drying method. Extrac- tion of aerial parts was done by perculator apparatus and using methanol 80%.Different alkaloids solvents such as pe- troleum and chloroform were added based on standard methods.The antimicrobial effect of methanolic extract and alkaloid extracts were evaluated against Staphylococcus aureus (PTCC 1431), Escherichia coli (PTCC 1399), Pseudomonas aeru- ginosa (PTCC 1430), Klebsiella pneumonia (PTCC 1053), Salmo- nella typhimurium (PTCC 1639), Candida albicans (PTCC 5027) based on CLSI 2012 protocol by disk diffusion meth- od.Further microdilution was done to determine Minimal inhibitory concentration(MIC).

Results: based on the results, only by microdilution method not disk diffusion, alkaloid extracts in 1000 mg/ml concen- tration inhibit the growth of E. …

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