Academic journal article Iranian Journal of Public Health

Study Role of Temperature Factor for Determine the Sensitivity of Common Yeast in the Mouth to Mouthwashes

Academic journal article Iranian Journal of Public Health

Study Role of Temperature Factor for Determine the Sensitivity of Common Yeast in the Mouth to Mouthwashes

Article excerpt

Background: The aim of the present study, investigation role of temperature factor for determine the sensitivity of common yeast in the mouth to mouthwashes

Methods: In the present study, a standard strain of Candida albicans PTCC 5027 was used as a common fungal yeast in the mouth. The effect of mouthwashes against the common yeast of mouth, C.albicans Evaluated by agar diffusion meth- ods. At first the suspension was provided by physiological saline and fresh culture of C.albicans (24 hours) and the OD was read at 530 nm wavelength by spectrophotometer. 10 µl of providing suspension was transferred to SDA medium. In the next step, two wells were made with suitable distance in culture medium, filling with. For decreasing the error, the test was repeated 3 times. Plates were placed in the incubator at 25 °C,30 °C, 37 °C for 24 hours.Inhibition zone was meas- ured and recorded by using a Collis. The data were analyzed by SPSS software (18th edition), using one sided variance analysis (ANOVA-one way) with P0. …

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