Academic journal article Iranian Journal of Public Health

Characterization of Leishmania Parasites in Suspected Patients of Cutaneous Leishmaniasis by Targeting Its-Rdna, Its-Microsatellite in Shush City, Northern Khuzestan (2012-2013s)

Academic journal article Iranian Journal of Public Health

Characterization of Leishmania Parasites in Suspected Patients of Cutaneous Leishmaniasis by Targeting Its-Rdna, Its-Microsatellite in Shush City, Northern Khuzestan (2012-2013s)

Article excerpt

Background: This study was done to identify the Leishmania parasites using microscopic and molecular methods in sus- pected patients of cutaneous leishmaniasis by targeting ITS- rDNA and ITS-microsatellite genes in Shush city, Northern Khuzestan.

Methods: 65 smears from confirmed patients' lesions of ZCL, in Shush city during 2012-13s, were taken, stained and examined under a light microscope. Whole DNA of parasites was extracted directly from prepared slides based on Phenol- Chloroform method and examined by polymerase chain reac- tion (PCR) of ribosomal DNA (rDNA) internal transcribed spacer (ITS) and ITS-microsatellite markers. Some positive amplicons were digested with BsuRI restriction enzyme ac- cording to RFLP method. In order to re-confirm, suspected PCR products were sequenced, edited and aligned by Se- quencher TM v. 4.1.4 software and exported to MEGA5.05 for phylogenetic analysis.

Results: 50 out of 65 and 57 out of 65 samples were detect- ed as Leishmania positive using microscopic and molecular methods respectively. …

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