Academic journal article Iranian Journal of Public Health

IDENTIFYING OF CAUSATIVE AGENTS OF CUTANEOUS LEISHMANIASES BY AMPLIFYING CYT B GENE IN INDIGENOUS FOCI OF Iran

Academic journal article Iranian Journal of Public Health

IDENTIFYING OF CAUSATIVE AGENTS OF CUTANEOUS LEISHMANIASES BY AMPLIFYING CYT B GENE IN INDIGENOUS FOCI OF Iran

Article excerpt

Background: In this study, simultaneously identifying of three causative agents species of CL is followed by targeting Cyt b gene in three regions of Turkmen Sahara, Ahvaz and Abarkouh.

Methods: Smears of suspected patients infected with zoono- tic cutaneous leishmaniasis (ZCL) were stained and examined under a light microscopic observation. DNA of parasites within human ulcers was extracted directly from their smears. PCR standard was used to amplify the Cyt b gene of Leishma- nia parasites in human from Turkmen Sahara, Ahvaz and Abarkouh. Based on RFLP method by digesting Ssp1 re- striction enzyme and more precisely sequencing of Cyt b was shown to be species-specific.

Results: In this investigation 290 of 310 confirmed patients were infected with L. major by having high density and low genetic diversity. Unlikely, 18 out of 310 cases were deter- mined to be L. tropica with low density and high diversity. Besides, for the first time L. turanica was isolated from two patients in Turkmen Sahara with low density rate and low molecular variation.

Conclusion: Results showed that at least three species of genus Leishmania are the causative agents of CL that are une- quivocally circulating in endemic foci of Iran. …

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