Academic journal article Iranian Journal of Public Health

Use of Lyophilized Rabbit Serum as Nutritional Replacement for the Foetal Calf Serum in Cultivation of Leishmania Major and Leishmania Infantum Promastigotes

Academic journal article Iranian Journal of Public Health

Use of Lyophilized Rabbit Serum as Nutritional Replacement for the Foetal Calf Serum in Cultivation of Leishmania Major and Leishmania Infantum Promastigotes

Article excerpt

Background: Genus Leishmania is a protozoan parasite that causes different severe diseases. Fetal Calf Serum (FCS) is the major part of the Leishmania culture media and the most ex- pensive ingredient in these media. In the present work, the nutritional efficacy of the lyophilized Rabbit serum was eval- uated in Leishmania culture media.

Methods: The efficacy of lyophilized Rabbit serum was evaluated by assassing the parasite growth and replication pattern after adding 5%-10% lyophilized Rabbit serum to the RPMI-1640 medium. The growth ability and behavior of promastigotes of Leishmanian parasites assessed at in vitro condition.

Results: According to our finding, 5% lyophilized Rabbit serum can be used for cultivation of Leishmania major and can support the growth of them. …

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