Academic journal article Iranian Journal of Public Health

Optimization of Bacillus Cereus Detection Based on Hemolysin Bl and Non Hemolytic Enterotoxin C Genes by Multiplex Pcr

Academic journal article Iranian Journal of Public Health

Optimization of Bacillus Cereus Detection Based on Hemolysin Bl and Non Hemolytic Enterotoxin C Genes by Multiplex Pcr

Article excerpt

Background: Bacillus cereus is a Gram-positive, facultative anaerobic, spore forming bacterium, which are widely spread in nature. B.cereus as a food-poisoning organism can cause two types of illness, the diarrhoeal form and the emetic form. B. cereus produces several toxins, including emetic toxin and at least four other enterotoxins: hemolysin BL or Hbl, nonhe- molytic enterotoxin or Nhe and two single proteins, cytotox- in K or CytK and enterotoxin FM or EntFM. This study investigates the molecular detection of Bacillus cereus by Multi- plex PCR and using hblA and nheC genes.

Methods: Isolateswere grown overnight at 37 °C on BHI broth, and purification of DNA was performed. The PCR reactions were performed for genes hblA and nheC, the PCR of those genes were optimized. …

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