Academic journal article Iranian Journal of Public Health

Recombinant Pcrv Induced Poly-Isotypic Humoral Immune Responses against Pseudomonas Aeruginosa in Balb/c Mice

Academic journal article Iranian Journal of Public Health

Recombinant Pcrv Induced Poly-Isotypic Humoral Immune Responses against Pseudomonas Aeruginosa in Balb/c Mice

Article excerpt

Background: The purpose of this study was to evaluate the feasibility of using PcrV as a candidate vaccine against Pseu- domonas aeruginosa and also to examine whether PcrV would promote the immune responses and induce poly-isotypic humoral immune response in burned mouse models.

Methods: Following the expression and purification of re- combinant PcrV (r-PcrV), immunization process was done, it must be reminded that, after each immunization course spe- cific total antibodies responses were measured to declare the efficacy of immunization. At the end different isotype- specific antibodies (IgG1, IgG2a, IgG2b and IgM) against r- PcrV measured with ELISA method. Four week after last immunization, Control (PBS injected group) and immunized (r-PcrV injected group)group of mice were burned and chal- lenged (subeschar) with approximately 5-3×102CFU (3 times LD50) of Pseudomonas aeruginosa PAO1.Survival rate and bac- terial quantity in skin and internal organs (Liver and Spleen) were evaluated 25 hour post infection to study systemic in- fection and reveal the pattern of immune responses.

Results: All immunized animals (rPcrV injected group) were able to produce specific antibodies against PcrV and highest antibody titer was detected after third immunization (second booster), Immunization with candidate vaccine (r-PcrV) sig- nificantly increased specific IgG1, IgG2a, IgG2b and IgM isotype antibodies compared with control group, (P<0. …

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