Academic journal article Iranian Journal of Public Health

USE OF MULTILOCUS SEQUENCE TYPING (MLST) IN MOLECULAR EPIDEMIOLOGY STUDY OF ANTIBIOTIC RESISTANCE PSEUDOMONAS AERUGINOSA ISOLATED IN Iran

Academic journal article Iranian Journal of Public Health

USE OF MULTILOCUS SEQUENCE TYPING (MLST) IN MOLECULAR EPIDEMIOLOGY STUDY OF ANTIBIOTIC RESISTANCE PSEUDOMONAS AERUGINOSA ISOLATED IN Iran

Article excerpt

Background: Nosocomial infections produced by multi- drug-resistant (MDR) Pseudomonas aeruginosa strains in Iran severely increased. Also nosocomial outbreaks of P. aeruginosa infection, particularly by MDR strains, have become prob- lematic in hospitals in various countries. The object of this study was to determine ST type of antibiotic resistance Pseu- domonas aeruginosa isolated in Iran

Methods: The real-time RT-PCR was performed to deter- mine the expression level of mexY, ampC and oprD for iso- lates. The isolates were typed by multilocus sequence typing (MLST) a strain-typing system that focuses strictly on con- served housekeeping genes. Also Antimicrobial Susceptibility testing was performed.

Results: 75% of clinical isolates were multidrug-resistant. The blaOXA group-I and blaPER alleles were identifiedin 28 and 10 P. …

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