Academic journal article Iranian Journal of Public Health

Isolation and Molecular Identification Free Living Nitrogen Fixing Bacteria from Vermicompost

Academic journal article Iranian Journal of Public Health

Isolation and Molecular Identification Free Living Nitrogen Fixing Bacteria from Vermicompost

Article excerpt

Background: many bacteria are able to fixing nitrogen, which is one of the magor nutrients essential for all living beings. The bacteria isolated from the soil can be used for various purposes.

Methods: In this research the samples were collected from 10 different cities of Iran and the preliminary screening tests such as gram staining, oxidase test,catalase test,nitrate test and some other tests were perforformed. The selected strains were tested by complementary screening tests such as cultur- ing in N-free malate agar media and those which had the highest ability to fixate nitrogen were selected.PCR and 16SrRNA Gene seguence analysis were used for molecular identificationn of superior strains.

Results: After enrichment and growth in Burk's N-free me- dium plates, 70 bacterial strains were isolated and by per- forming nitrate test 40 of them were recognized as nitrogen fixing strains. …

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