Academic journal article Iranian Journal of Public Health

Isolation and Molecular Identification of Native Rhodococcus Strains from Manganese Mine Soil in Qom City

Academic journal article Iranian Journal of Public Health

Isolation and Molecular Identification of Native Rhodococcus Strains from Manganese Mine Soil in Qom City

Article excerpt

Background: The aim of this study was isolation and mo- lecular identification of Rhodococcus native strains from soil samples of manganese mine in Qom city.

Methods: Totally 125 samples have been collected from soil around manganese mine in Qom city. The samples were seri- ally diluted (10 -1- 10-7) and cultivated on different selective media. Then the pure coloies were physiologically and bio- chemically determined and characterized at 30°C.Genomic DNA extraction and PCR amplification of the 16 SrRNA genes were done and aligned with the 16rsRNA gene se- quences of other Rhodococcus species obtained from Gene- Bank/EMBL/DDBJ.

Results: Overall, 125 bacterial strains were achieved after culturing on different selective enriched media such as ISP5 and Bennet agar and 87 strains were similar to the coryne form among them two strains (85, 87) which is isolated from ISP5 agar, were identified and confirmed by biochemical tests and 16srRNA PCR amplification method as Rhodococcus rhod- nii and Rhodococcus rhodocorrhous. …

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