Academic journal article Iranian Journal of Public Health

Parasporin, a Cytotoxic Protein against Human Breast Cancer Cell of Bacillus Thuringiensis

Academic journal article Iranian Journal of Public Health

Parasporin, a Cytotoxic Protein against Human Breast Cancer Cell of Bacillus Thuringiensis

Article excerpt

Background: Parasporin, a Bacillus thuringiensis parasporal protein, is unique in having a strong cytocidal activity prefer- ential for human cancer cells. In this study, we characterized parasporin activities against human breast cancer cell line.

Methods: Soil samples were collected and then isolates were identified using phenotypic and Cry toxin staining. In next step, toxin was separated, purified, and activated using pro- teinase-K enzyme. The toxin molecular weight was evaluated using SDS-PAGE method. Breast cancer cell line was cul- tured and then treated with activated toxin.

Results: B. thuringiensis strain Kla4 produced one parasporal inclusion proteins with a molecular mass of 37 kDa that was converted to 30 kDa toxin when activated by proteinase K digestion. This toxin exhibited strong cytocidal activity against human breast cancer cell, while the isolate lacked lymphocyte toxicity and hemolytic activity against sheep erythrocytes. …

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