Academic journal article Iranian Journal of Public Health

Isolation and Screening of Native Streptomyces Strains Able to Production of Biosurfactant from Saline Soil

Academic journal article Iranian Journal of Public Health

Isolation and Screening of Native Streptomyces Strains Able to Production of Biosurfactant from Saline Soil

Article excerpt

Background: Objective of the present study is isolation and screening of biosurfactant producing Streptomyces from saline soil in Qom city.

Methods: Totally 40 soil samples were collected from differ- ent regions of saline lake in Qom city and were dilluted ( 10 - 1- 10-7) and cultivated on different media including: starch casein agar and ISP4 medium. The plates were incubated at 30°C for 3-7 days and then the pure isolates were identified due to biochemical and morphological tests. Biosurfactant activity was determined for the pure culture of Streptomyces isolates by different methods namely: 1) hemolysis 2) drop collapsing test 3) oil spreading 4) lipase production 5) surface tension measurement.

Results: In the serial dilution and plating on starch casein agar about 95 Actinobacterial strains were isolated. Among the isolated bacteria 25 strains belonged to Streptomyces genus. In general, six isolates were capable of biosurfactant produc- tion. Morphologically distinct colonies were selected for fur- ther analysis. …

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