Academic journal article Iranian Journal of Public Health

The Effect of Silver Nonoparticles on Staphylococcus Epidermidis Biofilm

Academic journal article Iranian Journal of Public Health

The Effect of Silver Nonoparticles on Staphylococcus Epidermidis Biofilm

Article excerpt

Background: Background and aims: Biofilm is a functional consortium of microorganisms attached to the surface and is embedded in the extracellular polymeric substances (EPS) produced by the microorganisms. Biofilm, due to the special structure and EPS are more resistant to antimicrobial agents. The purpose of this study was to investigate the effect of silver nonoparticles on Staphylococcus epidermidis.

Methods: Methods: Bacteria were isolated from different patients' samples referred to Mashhad hospitals and identi- fied by biochemical tests. Nanoparticles with a diameter of 20 nm were purchased from Nanosany Company (USA). Mini- mum inhibitory concentration (MIC) and minimum bacteri- cidal concentration (MBC) of nonoparticles were determined by using agar dilution method in 24- well micro titer plate. 96- Well micro titer plate and ELISA reader were used for evaluating the effect of silver nanoparticles on the biofilm of Staphylococcus epidermidis. Biofilms were stained with safranin.

Results: Results: Silver nanoparticles showed antibacterial activity on 15 clinical isolates and the reference strain of S. …

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