Academic journal article Iranian Journal of Public Health

The Effect of Garlic Juice on the Growth and Extra Cellular Protein Profile of Methicillin- Resistant Strains of S.aureus

Academic journal article Iranian Journal of Public Health

The Effect of Garlic Juice on the Growth and Extra Cellular Protein Profile of Methicillin- Resistant Strains of S.aureus

Article excerpt

Background: Staphylococcus aureus is an important etiologic agent of food-borne diseases due to its ability to produce heat-resistant staphylococcal enterotoxins (SEs) when it grows in foods and also the rise in antibiotic resistance has further amplified public health concern. Because of im- portance of staphylococcal exotoxines and exoenzymes in Staphylocoous aureus pathogenesis, in the present study, we examined the effect of garlic juice on growth and extracellu- lar proteins production of methicillin-resistant Staphylococcus aureus.

Methods: A total of 20 methicillin resistant S.aureus isolated from different hospitals in Rasht was determined by using Kirby bauer disc diffusion technique. Antimicrobial activity of fresh garlic juice against test bacteria was evaluated by using agar well diffusion. Minimum inhibitory and minimum bactericidal dilution of garlic juice were determined using the microdilution method. To verify the inhibitory effect of garlic juice on extracellular proteins production, the staphylococcal cells from an BHI overnight culture, containing different concentrations of garlic juice, were harvested by centrifuga- tion and separated by polyacrylamide gel electrophoresis. …

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