Academic journal article Iranian Journal of Public Health

Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus in Hospital-Related Healthy Carriers in Mofid Children Hospital

Academic journal article Iranian Journal of Public Health

Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus in Hospital-Related Healthy Carriers in Mofid Children Hospital

Article excerpt

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main causes of nosocomial diseases in hospitalized children. Then, screening healthy carriers of these bacteria who are in direct contact with patients in hos- pital is nesceccery.

Methods: Two hundreds tweny nine medical staff were ex- amined and nasal samples for S. aureus culture and sociodem- ographic data were obtained from them during one year, 2012-2013. After screening for oxacillin and cefoxitin sus- ceptibility, all isolates were examined for antimicrobial sus- ceptibility pattern and staphylococcal cassette chromosome mec (SCCmec) type I-V genes.

Results: From 229 tweny nine nasal samples examination, 27 Staphylococci were isolated and 21 of them were Methicillin- resistant Staphylococcus aureus( MRSA). The highest prevalence resistant to antibiotics were: penicillin (90.3%), ceftazidime(77.4%), aztreonam (79%). Resistant to trime- toprime-sulfametoxazol (19%), doxycyclin(29%), minocyclin (12%), rifampicine(16%) were not high. All isolates sensitive to linezolid and vancomycin. Of those tested, 4 isolates was nontypable by using the published primers, perhaps indicat- ing the existence of a novel SCCmec class. …

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