Academic journal article Iranian Journal of Public Health

Correlation of Macroscopic Biofilm Production and Presence of Virulence Determinants in Clinical Isolates of Staphylococcus Epidermidis

Academic journal article Iranian Journal of Public Health

Correlation of Macroscopic Biofilm Production and Presence of Virulence Determinants in Clinical Isolates of Staphylococcus Epidermidis

Article excerpt

Background: Ability of biofilm production is one of the molecular mechanisms of S. epidermidis pathogenesis. Viru- lence related gens play role in the bacterial attachment to artificial surface and host surfaces which covered by proteins. This study was investigated the correlation of icaA, aap, fbe, bhp and IS256 presence and ability of macroscopic biofilm production in S. epidermidis clinical isolates.

Methods: Macroscopic biofilm production assay has been done by microtiter plate method. Bacteria were cultured in TSB medium included NaCl and glucose, pour in polystyrene well, incubated in 370C, washed with P.B.S, stained by safra- nin, and then the OD measured in 490 nm. S. epidermidis virulence-associated genes were detected by specific PCRs.

Results: Ability of macroscopic biofilm production was ob- served in 61% of isolates by microtiter plate method. …

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