Academic journal article Iranian Journal of Public Health

Study of Biofilm Formation and Detection of the Icaadbc Gene Cluster among Clinical Isolates of Staphylococcus Aureus and Staphylococcus Epidermidis in Shiraz Teaching Hospitals, 2013

Academic journal article Iranian Journal of Public Health

Study of Biofilm Formation and Detection of the Icaadbc Gene Cluster among Clinical Isolates of Staphylococcus Aureus and Staphylococcus Epidermidis in Shiraz Teaching Hospitals, 2013

Article excerpt

Background: This study aimed to determine biofilm pro- ducing ability and presence of icaAD gene in clinical staphy- lococcal isolates as well as to assess the reliability of two phe- notypic methods used for detection of biofilm.

Methods: This study was performed on 151 staphylococcal isolates (79 and 72 isolates of Staphylococcus aureus and Staphylo- coccus epidermidis, respectively) obtained from different clinical specimens during a 5 months period in 2013 from four Shi- raz Teaching Hospitals. Slime production assay was per- formed by cultivation on Congo Red Agar (CRA). Quantita- tive biofilm formation was assessed by microtiter plate assay (MPA) and PCR was used for detection of icaAD gene. In addition, antibiotic susceptibility profiles were determined by the disc diffusion method according to CLSI guidelines.

Results: Of the S. epidermidis isolates, 71% were CRA posi- tive, 76% adherence positive using the MPA and 75% carried the icaAD gene. Among S. aureus isolates, 54%, 58% and 63% were CRA, adherence and icaAD positive, respectively. …

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