Academic journal article Iranian Journal of Public Health

ASSOCIATION BETWEEN PRODUCTION OF THE ENTEROTOXIN A AND THE PRESENCE OF COA AND NUC GENES AMONG S. AUREUS ISOLATED FROM VARIOUS SOURCES, IN SOUTHERN Iran

Academic journal article Iranian Journal of Public Health

ASSOCIATION BETWEEN PRODUCTION OF THE ENTEROTOXIN A AND THE PRESENCE OF COA AND NUC GENES AMONG S. AUREUS ISOLATED FROM VARIOUS SOURCES, IN SOUTHERN Iran

Article excerpt

Background: Staphylococcus aureus is considered as a common cause of food-borne disease, worldwide. The present study was aimed to identify the frequency of coagulase (Coa) and thermonuclease (Nuc) genes and Staphylococcal enterotoxin A (Sea) production among S. aureus isolated from various sources in Shiraz. Moreover, the correlation between the Sea gene and coagulase and thermonuclease enzymes is also con- sidered.

Methods: A total of 100 S. aureus isolated from various sources including 40 humans, 30 animals and 30 food sam- ples by the routine biochemical tests. The frequency of Coa, Nuc and Sea genes was evaluated by PCR assay. The entero- toxin production was confirmed using SDS-PAGE method. Correlation among those genes was finally evaluated by sta- tistical analysis.

Results: The PCR results showed that the prevalence of Coa, Nuc and Sea genes were 91%, 100% and 14%, respec- tively. …

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