Academic journal article Iranian Journal of Public Health

Distribution of the Cyanide-Resistant Alternative Oxidase in the Fungal Class of Eurotiomycetes (Ascomycota, Pezizomycotina)

Academic journal article Iranian Journal of Public Health

Distribution of the Cyanide-Resistant Alternative Oxidase in the Fungal Class of Eurotiomycetes (Ascomycota, Pezizomycotina)

Article excerpt

Background: Here we investigated the distribution of this enzyme within the fungal class of Eurotiomycetes.

Methods: 111 AOX protein sequences were mined from various nucleotide databases available at the National Center for Biotechnology Information (NCBI) and the Joint Ge- nome Institute (JGI). Gene models and products were de- duced manually. The resultant peptidic sequences were aligned with MAFFT version 7 using the E-INS-i algorithm and a BLOSUM 30 similarity matrix. Curation was carried out with Block Mapping and Gathering using Entropy (BMGE) with a BLOSUM 45 similarity matrix. A maximum likelihood tree was then obtained with PhyML and drawn with FigTree.

Results: Half of the sequenced Aspergillus and all Pencillium species specify a second alternative oxidase, which is likely to have evolved within the Aspergillaceae family (Eurotiales order) by duplication before the divergence of the two gene- ra. Two species (A. sydowii and P. brevicompactum) even have three isoenzymes. The second enzymes in Aspergillus clavatus and monascus are however, related to Dothideomycetes enzymes possibly suggesting gene transfer events. Another horizontal gene transfer involves the second AOX in Bysso- chlamys spectabilis (Thermoascaceae, Eurotiales) positioned at the basis of the Sordariomycetes clade. …

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