Academic journal article Iranian Journal of Public Health

Molecular Typing of Waterborne Escherichia Coli by Rep-Pcr

Academic journal article Iranian Journal of Public Health

Molecular Typing of Waterborne Escherichia Coli by Rep-Pcr

Article excerpt

Background: Molecular typing is a valuable tool for investi- gation of genetic relationship between the microbial strains. The aim of the current study was to determine the molecular types of waterborne E. coli strains using Rep-PCR.

Methods: Current study included one hundred waterborne E. coli strains isolated from different water sources in Karaj in 2013. Bacterial isolates were detected and identified by stand- ard microbiological and biochemical tests. Genomic DNA was extracted by AccuPrep® Genomic DNA Extraction Kit and genetic relationship between the strains was evaluated by Rep-PCR using specific primers. PCR amplicons were visual- ized after electrophoresis and staining with ethidium bromide and dendogram was constructed based on Dice Comparison method and UPGMA Clustering.

Results: Using Rep-PCR, all strains were typeable. More than fifteen different bands ranging from 130 to 2300 bp were amplified in different profiles. …

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