Academic journal article Iranian Journal of Public Health

Determination of Uropathogenic and Fecal E.coli O Serotypes Isolated from Zabol by Multiplex Pcr

Academic journal article Iranian Journal of Public Health

Determination of Uropathogenic and Fecal E.coli O Serotypes Isolated from Zabol by Multiplex Pcr

Article excerpt

Background: Escherichia coli(E.coli) can be serotyped by de- termination of O antigens, and some relationship exists be- tween specific O types and pathogenic behavior. There are rare information about the prevalence of O serogroups of uropathogenic and fecal E.coli isolates in Iran. This study was performed to determine the distribution of the 12 principal O-Serotypes among uropathogenic and fecal E.coli .

Methods: In total, 194 E. coli isolates collected from hospital patients of the Zabol hospitals (Zabol, Iran) were studied: 100 uropathogenic and 94 fecal E. coli isolates. Confirmation of E.coli samples were done by biochemical tests. The identi- fication of O-serogroups(O1, O2, O4, O6, O7, O12, O15, O16, O18, O25, O75 and O157) were performed by Multi- plex PCR method.

Results: Among uropathogenic and fecal E.coli, 73 (73%) and 67(71.27%) were serotyped successfully by multiplex PCR, respectively. In this study was determined that O2, O6 and O18 Serogroups in uropathogenic E. …

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