Academic journal article Iranian Journal of Public Health

Investigation of Tigecycline Resistance among Metallo-Beta-Lactamase Producing Acinetobacter Baumannii Isolates in an Educational Hospital of Esfahan

Academic journal article Iranian Journal of Public Health

Investigation of Tigecycline Resistance among Metallo-Beta-Lactamase Producing Acinetobacter Baumannii Isolates in an Educational Hospital of Esfahan

Article excerpt

Background: This study was designed to investigate the tigecycline resistance among metallo-beta-lactamase produc- ing Acinetobacter baumannii isolates in an educational hospital of Esfahan.

Methods: In this cross-sectional study, 107 A. baumannii recovered from trachea, bronchial, wound urine; were identi- fied by standard biochemical tests; and conformed as Acineto- bacter baumannii species using blaOXA-51- like gene and PCR method. Antimicrobial susceptibility to 15 antibiotics was performed using Kirby-Bauer disc diffusion method; also, strains of A. baumannii non-susceptible to imipenem were screened for MBL production. Determination of minimum inhibitory concentration to colistin sulfate was carried out using E-test method. Combination disk was used for detec- tion of MBL production. A solution was prepared by dissolv- ing 186.1 g of disodium EDTA.2H2O in 1,000 mL of dis- tilled water and adjusting it to PH 8.0. Two 10 µg imipenem disks were placed on the plate, and 10 µl of a 0.5 M EDTA solution was added to one of them. Increasing of inhibition zone with imipenem-EDTA ≥ 5 mm was determined as MBL producing isolate. According to EUCAST interpreta- tive criteria, diameter of inhibition zone ≥18 mm sensitive and 15> mm resistant were considered.

Results: Totally, 100 isolates (93.45%) were MBL producers. …

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