Iranian Journal of Public Health

Provides articles in all areas of health.

Articles from Vol. 43, No. 2, 2014

A–P P–U
A Comparative Study of Antimicrobial Activity of Nisin Loaded in Alginate and Alginate-Resistant Starch Microparticles
Background: This study was designed to compare antimi- crobial activity of alginate and alginate-resistant starch micro- particles against Listeria monocytogenes.Methods: Nisin loaded microparticles were prepared by a w/o-emulsion external cross-linking...
A COMPARATIVE STUDY ON DIAGNOSTIC ASSAYS, PCR, MICROSCOPIC AGGLUTINATION TEST (MAT) AND CULTURE FOR DETECTION OF RODENT LEPTOSPIROSIS IN MAZANDARAN PROVINCE, Iran
Background: Leptospirosis is caused by infection with Lep- tospira bacteria. The infection transfers from animals (com- monly rats, cattle, pigs and dogs) to humans. Transmission occurs by direct or indirect contact with serum, urine or oth- er biological...
A COMPARISON AMONG VARIOUS SELECTIVE MEDIA TO ISOLATE THE NOVEL HEAT RESISTANT ACTINOMYCETES FROM SOIL SAMPLES OF GANDOMBERIAN AREA OF THE LUT DESERT, Iran
Background: The aim of this study was to evaluate different selective media in the isolation of the heat resistant actino- mycetes.Methods: 46 samples were collected from the surface (sur- face to 5cm depth) and depth (5-20cm depth) soils of three identified...
Analysis of Single Nucleotide Polymorphisms (Snps) in Pertussis Toxin Promoter of Vaccine and Clinical Isolates of Bordetella Pertussis
Background: The aim of this study is to identify the single nucleotide polymorphisms (SNPs) of pertussis toxin promot- er (ptxP) in clinical isolates and vaccine strains.Methods: Five clinical isolates of Bp, Tohama I as the refer- ence strain, and 2...
Analytical Specificity and Sensitivity of Loop Mediated Isothermal Amplification Assay Targeting Com1 Gene of Coxiella Burnetii
Background: C.burnetii, the bacterium causing Q fever, is an obligate intracellular biosafety level 3 agent. Detection and quantification of these bacteria with conventional methods is time consuming and dangerous. Rapid detection of the agent improves...
Antibacterial Effect of Silver Nanoparticles on Acinetobacter Baumannii
Background: Acinetobacter baumannii is a Gram-negative, ob- ligate anaerobic, non-fermented and non-motile bacterium. Acinetobacter baumannii has become a particularly important organism in the Intensive Care Unit (ICU). Acinetobacter bau- mannii plays...
ANTIBIOTIC RESISTANCE OF BACTERIA ISOLATED FROM CLINICAL SPECIMENS IN A UNIVERSITY HOSPITAL IN TEHRAN, Iran
Background: Determination of antibiotic resistance of clini- cal isolates can be helpful for delegacy of appropriate drug in urgent experimental remedy of infections.Methods: We tested antibiotic resistance of 696 non- duplicate clinical isolates were...
ANTIBIOTIC SUSCEPTIBILITY PROFILE OF COMMON BACTERIAL PATHOGENS IN THE PEDIATRIC WARD OF DASTGHEIB HOSPITAL, SHIRAZ, Iran: A CROSS SECTIONAL STUDY
Background: The aim of this study was determination of etiological agents of bacterial infection and their antimicrobial susceptibility profiles in the pediatric ward of Dastgheib hos- pital, Shiraz.Methods: A cross-sectional study was carried out on...
Anti-Listerial Activity of Microencap-Sulated Nisin in Different Biopolymers Microparticles
Background: The aim of this study was to evaluate the anti- listerial activity of nisin microencapsulated in three polymeric systems including alginate, alginate-resistant starch and algi- nate-high methoxy pectin.Methods: Nisin loaded microparticles...
ANTIMICROBIAL ACTIVITY OF ENDOPHYTIC BACTERIAL POPULATIONS ORIGINATED FROM MEDICAL PLANTS IN Iran
Background: The aim of this study was the isolation, mo- lecular identification and antimicrobial activity of endophytic bacteria isolated from medicinal plants in some areas of Iran.Methods: From April to Jun 2013 and April to May 2014, 23 fresh plant...
Antimicrobial Effects of Zinc Oxide and Silver Nitrate Nano Particles on S.aureus, A.baumannii and P.aeroginosa
Background: Nano particles have been introduced as novel antimicrobial agents because of their properties that are dif- ferent from their bulk properties. Present study is aimed to investigate antimicrobial activity of silver nitrate and zinc oxide nano...
Antimicrobial Resistance and Survey of Pathogenic Genes of Stx1 and Stx2 in E.coli O157: H7 Isolates from Beef Cattle at Industrial Slaughter House of Fars Province with Multiplex Pcr Method
Background: E. coli O157: H7 is one of the most important pathogenic bacteria that produce, hemorrhagic colitis in hu- mans. Livestock are the main reservoir of these bacteria. The aim of this study was to determine and evaluate the antibiotic resistance...
Antimicrobial Susceptibility of Bacterial Pathogens Causing Urinary Tract Infection in Besat Hospital of Sanandaj, 2012-2013
Background: The present study aimed to ascertain the cur- rent situation of antimicrobial resistance of Urinary Tract Infections (UTIs) caused by human pathogens in the Besat hospital of Sanandaj to provide data to help direct empirical therapyMethods:...
ANTI-TRICHOMONAS VAGINALIS ACTIVITY OF THE FOENICULUM VULGARE FROM IranIAN SEMI-ARIED REGION
Background: Trichomonas vaginalis is a parasitic protozoan with a predilection for human urogenital tract and causative agent for vaginitis, cervicitis and urethritis in females. The drug of choice for therapy is metronidazole however the main drawback...
Bacterial Contamination of Traditional Ice Cream in Tehran
Background: From hygienic quality aspect, most of the pre- sent traditional ice cream has non-conforming with extant standards. Pasteurization of primary ice cream mixture with sufficient thermal processing increase hygienic quality level and causes...
Bioremediation of Nickel through Metarhizium Sp. Utmc 5002
Background: In this study, we isolated nickel resistance fun- gi and evaluated their potential for bioremediation appli- cation in acidic pH condition.Methods: Soil samples were collected from heavy metal con- taminated parts of Iran. Fungal colonies...
CHARACTERIZATION OF OVERT AND OCCULT HEPATITIS B VIRUS INFECTION AMONG HTLV-1 POSITIVE HEALTHY CARRIERS IN THE NORTHEAST OF Iran; AN HTLV-I ENDEMIC AREA
Background: To date, no studies have provided data on hepatitis B virus (HBV) prevalence among asymptomatic, healthy human T-lymphotropic virus (HTLV-I) positive car- riers. This sero- and molecular epidemiology study was per- formed on patients in the...
Citrinin Reduction in Wheat Flour by Using "Yeast Saccharomyces Cerevisiae"
Background: Wheat is the main cereal that is used in Iran and in the world. Citrinin is a toxic metabolite produced by Penicillium, Aspergillus and Monascus into grains, foods, feedstuffs. Eating this contaminated product causes of acute and chronic...
Comparative Study of of Antimicrobial Activities of Cu and Zno Nanoparticles against the Pathogenic Strain of Klebsiella Pneumoniae
Background: The use of metal nanoparticles can be effec- tive to eliminate the bacterial infections, as an alternative to antibiotics. The aim of this study was to detect the antibacte- rial properties of 0.01, 0.5 and 1% nano-Cu and nano-Zno against...
COMPARISON BETWEEN GENE CASSETTES ARRAY OF INTEGRON CLASS I IN MULTIDRUG RESISTANCE ACINETOBACTER BAUMANNII AND KLEBSIELLA PNEUMONIAE IN Iran
Background: Integrons are considered to play a major role in multidrug resistance dissemination among gram-negative bacteria. These are genetic elements capable of integrating and mobilizing individual gene cassettes via a site-specific recombination...
Comparison of Cage and Baba Virulence Genes Expression in Spiral and Coccoid Forms of Helicobacter Pylori
Background: Under stressful environment, a spiral form of H. pylori is transformed into a coccoid form. The coccoid formation is very important problem in recurrence of disease and antibiotic treatment failure in gastritis pateints. Our ob- jective of...
Continuous Screening of Lipid Degradating Yeasts and Its Application in Treatment of Oily Wastewater
Background: The aim of this study was to continue the screening of lipophilic yeasts with high specific growth rate for wastewater treatment.Methods: Soil samples were collected from soil with oil pol- lution. These samples were used in a continuous...
Detection of Chlamydia Trachomatis in Fertile and Infertile Women in Sanandaj by Pcr
Background: The aim of this study was to determine the prevalence of C. trachomatis in infertile and fertile women in Sanandaj (Kurdistan, Iran).Methods: 104 infertile women referring to Clinic-affiliated Besat hospital in Sanandaj (Kurdistan, Iran)...
Detection of N-Acyl Homoserine Lactone (Ahl) in Biofilm Producing Uropathogenic Escherichia Coli Isolated from Urinary Tract Infection (Uti) Samples
Background: N-Acyl homoserine (AHL) is the main com- ponent in quorum sensing and play an important role in bio- film formation. Little information is available regarding to AHL in E.coli. The purpose of this investigation was detec- tion of AHL production...
Determination the Frequency of Aders Genes among Clinical Isolates of Carbapenem Resistance A. Baumannii Strains
Background: Carbapenems are considered as the key treat- ment in many cases of nosocomial infections of Acinetobacter baumannii. AdeABC efflux pump is the first characterized resistance-nodulation-cell division (RND) systems in re- sistant A. baumannii...
Development a Taqman Real-Time Pcr Assay with an Internal Positive Control for Quantitative Detection of Neisseria Meningitides
Background: This study was development a TaqMan Real- time PCR assay for rapid and specific quantitative detection of N. meningitidis. Also, by designing and using an Internal Positive Control (IPC) to monitor the assay, we were able to distinguish the...
Different Phenotypic Aspects with No Genotypic Heterogeneity in Leishmania Major Isolates of Suspected Patients in Northern Khuzestan Province
Background: In this inquiry, behavioral aspects of L.major isolates are evaluated in two forms of genotypic and phenotypic characters among suspected patients of some exceptional zoonotic cutaneous leishmaniasis (ZCL) locations in northern Khuzestan,...
Direct Identification of Streptococcus Agalactiae at Vaginal Colonization in Pregnant Women by Pcr
Background: Streptococcus agalactiae is a commensal organism but it may cause infection in susceptible hosts including new- borns, pregnant or postpartum women. Applying rapid, accu- rate, and sensitive method for detecting GBS and receiving intrapartum...
Distribution of Main E. Coli Phylogroups Encoding Ctx-M Esbl in Fecal Isolates of Poultry: A Surveil-Lance Study in Five Poultry Houses in Tehran
Background: Association of avian-E. coli phylogroups with human diseases and role of these strains in dissemination of resistance genes to human ones are of major health concerns. This study was aimed to investigate phylogroup diversity and prevalence...
Distribution of Main E. Coli Phylogroups Encoding Ctx-M Extended- Spectrum Beta-Lactamase (Esbls) in Fecal Isolates of Poultry: A Surveillance Study in Five Poultry Houses in Tehran
Background: Association of avian E. coli phylogroups with human diseases and role of these strains in dissemination of resistance genes to human ones are of major health concerns. This study was aimed to investigate phylogroup diversity and prevalence...
Distribution of the Cyanide-Resistant Alternative Oxidase in the Fungal Class of Eurotiomycetes (Ascomycota, Pezizomycotina)
Background: Here we investigated the distribution of this enzyme within the fungal class of Eurotiomycetes.Methods: 111 AOX protein sequences were mined from various nucleotide databases available at the National Center for Biotechnology Information...
Distribution of the Subtilase Cytotoxin Gene (Suba) among Shiga Toxin-Producing Escherichia Coli Isolated from Different Sources
Background: Subtilase cytotoxin is a potent AB5 toxin that first discovered in O113: H21 Shiga toxin-producing Esche- richia coli (STEC) strain involving in an outbreak of hemolytic uremic syndrome (HUS) in Australia and introduced in 2004 as a novel...
DNA Extraction of Malaria Parasites from Blood Slides
Background: Different factors should be present for having good PCR results, among them the quality and quantity of template DNA has more importance. The blood is the com- monest biological sample especially for malaria diagnosis. Extraction of DNA from...
Effect of Efflux Pump Inhibitor Phenylalanine- Arginine B-Naphthylamide on the Minimum Inhibitory Concentration of Imipenem in Mdr Acinetobacter Baumannii Isolated from Burnt Patients
Background: Efflux pumps of the RND super family such as AdeABC play a significant role in producing multidrug resistance A.baumannii as important agents in burn patients. So, the aims of this study were determination the role of ef- flux pump, effect...
Effect of Long-Term Exposure of Candida Albicans to Mobile Phone Radiation on Its Aint1 Gene Sequence
Background: In this study, for the first time, the mutagenic impact of long-term exposure to 900-MHz radiation on Can- dida albicans alpha-Int1 gene sequences were evaluated.Methods: A standard EMF generator with output ranging from 860 to 940 MHz was...
Effect of Salt Resource on the Halobacterium Salinarum Growth in Fed-Batch Culture with Aeration
Background: Halophilic Archaea based on structural charac- teristics, physiological, biochemical and genetically distinct from other microorganisms has attracted the attention of scientists. Halobacteriumsalinarum produces a membrane em- bedded photoprotein...
Efficiency of Lactic Acid Bacteria in Biological Control against Fusarium Sp. in Corn Field in Gorgan Area
Background: Soil-borne fungi such as Fusarium are plant pathogens like cereals and other crops. Since disabling this, funges and their produced mycotoxins by physical and chem- ical methods are costly and destroy nutrients, so biological detoxifications...
Elimination of Bacterial and Fungal Contaminations from Microalgal Culture
Background: The use of antibiotics is one of the means of dealing with contamination, but it should be noted that Chlo- rella is eukaryotic and anti-fungal compound might have neg- ative effects on it.Methods: The alga strain used in this experiment...
Enrichment, Isolation and Identification of Mtbe- Degrading Bacterial from Wastewater Mtbe Plant in Mahshahr
Background: The aim of this work was to isolate and identi- fy soil microorganism capable of degrading MTBE.Methods: Samples collected from a wastewater MTBE plant of Mahshahr Petrochemical, Iran, and carried in screw-cap bottle to laboratory. Enrichment...
Enterotoxaemia Caused by Clostridium Perfringens Type a in Gazelle
Background: Clostridium perfringensis a gram-positive anaero- bic bacterium that is divided into five subtypes. All subtypes produce alpha toxin. In addition, type C produces beta toxin and type D produces epsilon toxin. Type A strains producing only...
EPIDEMIOLOGICAL SITUATION OF BRUCELLOSIS IN THE PAST THREE DECADES IN Iran
Background: Brucellosis is one of the most common infec- tious diseases in Iran, Middle East and the vast areas world- wide. As Brucellosis is transmitted from animals to humans (Zoonoses), in addition to debility of patients and complica- tions from...
Escherichia Coli and Shigella Genus Detection in Water Sample by Pentaplex Pcr
Background: The aim of this study was to determine the most ubiquitous Escherichia coli specific genes to apply as a genetic marker to speed up the detection time and the sensi- tivity and introduce a multiplex PCR to detect and screen this marker organism...
Evaluation and Selection of Additives for Bioremediation of Petroleum-Contaminated Soil Using Oil-Degrading Bacteria
Background: As the release of petroleum hydrocarbons into the environment cause harmful effects to ecosystem and hu- man life, treating this contamination by applying sophisticat- ed and economical methods such as bio-remediation is nec- essary and important.Methods:...
Evaluation of Antimicrobial Activity of Green Tea Extract on Bacteria Isolated from Dental Plaque
Background: Green tea is one important factors to inhibi- tion of growth of oral bacteria like Streptococcus mutans and Enterococcus faecalis.This study investigates the antibacterial effect of green tea extract,on Enterococcus faecalis and Strepto-...
Evaluation of Bacterial Tolerance to the Zinc Metal in the Phase 1 Input of Wastewater Treatment Plant of Shahinshahr, Isfahan
Background: Agricultural and industrial activities generate wastewater containing heavy metals such as Zinc, which can be a major problem for the ecosystem and human health. Today, the use of microbial biomass for removal of heavy metals from industrial...
Evaluation of Crude Oil Biodegradation and Laccase Activity in Newly Isolated Phaeosphaeria Sp Utmc 5003
Background: The present study was carried out to investi- gate Iranian indigenous fungal strains to remove crude oil pollutants.Methods: First, for the fungal strain isolation, the contami- nated soil samples were collected from different habitats of...
EVALUATION OF CURING AGENTS IN PSEUDOMONAS AERUGINOSA ISOLATES FROM BURNED PATIENTS IN ISFAHAN, Iran
Background: The present study was conducted to evaluate the antibiotic resistant occurrence of plasmid and to compare the curing agents in Pseudomonas aeruginosa isolated from wound of burned patients.Methods: 220 clinical samples were collected from...
Evaluation of Microbial Contamination Automated Teller Machines (Atms) in Hamadan City
Background: Bacteria are ubiquitous organisms among the creatures that exist everywhere in the environment which able to grow on any surface. Continuous development and expansion of urbanization with increasing rate of population, and also limited times,...
Evaluation of Microbial Life in Salt Lake Razazeh, Karbala - Iraq
Background: The aim of this research was to evaluate halo- philic bacterial diversity in Lake Razazeh as regards this is the first report on halophilic bacteria isolation in Iraq.Methods: Samples of saline soils, mud and water were col- lected from 15...
Evaluation of Pyrite Removal from Mouteh Refractory Gold Ore Using Acidithio Bacillus Ferrooxidans Ptcc1647 in Semi Industrial Scale
Background: The aim of this work is to evaluate the pyrite remove from mouteh gold ore by A. ferrooxidans PTCC1647 in column.Methods: In the first step, Acidithio Bacillus Ferrooxidans PTCC1647 purchasad from (Iranian Research Organization for Science...
Evaluation of Resistance Pattern of Pneumococcal Pneumonia to Ceftriaxone, Azithromycin and Co-Amoxiclav in Clinic and Laboratory: A Trial Study
Background: Streptococcus pneumoniae is the most common cause of bacterial infections in the respiratory system. In re- cent years in addition to the high incidence of pneumococcal resistance to penicillin, resistance to other antibiotics has also emerged....
Evaluation of the Analytical Sensitivity of Loop-Mediated Isothermal Amplification (Lamp) Method for Rapid Detection of Extended Spectrum Beta Lactamases, Ctx-M-1 Family
Background: In this study LAMP, a new molecular tech- nique was evaluated for detection of CTX-M 1 gene.Methods: Sequences of related gene of CTX-M-1 family were retrieved from Gene Bank and aligned. By using of the consensus sequence, LAMP specific...
EVALUATION OF THE BACTERIA RECOVERED FROM VAGINAL SWABS IN WOMEN OF PARS HOSPITAL IN TEHRAN, Iran
Background: The isolation of vaginal bacteria from females indicates colonization or infection and their complexity are influenced by sexual activity, childbirth, tampon use and oth- er happenings during their reproductive life. The objective of this...
EVALUATION OF THE GENETIC SIMILARITY OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED FROM IranIAN AND AFGHAN TB PATIENTS USING RFLP-BASED METHODS
Background: Tuberculosis is an old problem that is current- ly considered a great challenge. Noticing Iran's borders with Afghanistan and Pakistan, which are among the 22 high bur- den countries around the world. this study awareness of pos- sible genetic...
Evaluation of the High Level Resistance to the Aminoglycoside in Enterococcus Faecalis by the Multiplex-Pcr Method
Background: High-level gentamicin resistance (MIC = 500 mg/L) is commonly intermediated by the aac (6')-Ie-aph (2 '')-Ia gene, which codes the bifunctional enzyme AAC (6 ')- APH (2 ''). In the recent years, three new aminoglycoside resistance genes [aph...
Extended-Spectrum Beta-Lactamase Producing Klebsiella Pneumoniae Isolates from Patients with Cystic Fibrosis
Background: The aim of this research was to determine the antibiotic resistance profiles as well as the prevalence of ß- lactamase production in K. pneumoniae isolates from CF pa- tients in Tehran.Methods: A total of 16 non-repetitive K. pneumoniae strains...
FREQUENCY OF ADHESIVE VIRULENCE FACTORS IN CARBAPENEMASE-PRODUCING ACINETOBACTER BAUMANNII ISOLATED FROM CLINICAL SAMPLES IN WEST OF Iran
Background: Acinetobacter baumannii is a significant oppor- tunistic pathogen which causes severe infections related to catheters and ventilator. Adhesive virulence factors (VFs) are effective in A. baumannii adherence and pathogenicity. The aim of this...
Frequency of Erma,b,c Genes in Erythromycin Resistant Enterococci Isolated from Samples of Hospitalized Patients in University Hospitals Qazvin & Tehran Cities
Background: Enterococci are among the most important caus- es of healthcare associated infections, especially urinary tract infections with multiple drug resistance. The main mecha- nism of erythromycin resistance in Enterococci is 23SrRNA methylation,...
GENETIC BACKGROUND OF URINARY ESCHERICHIA COLI STRAINS AND FLUOROQUINOLONE RESISTANCE IN SELSELEH AND DELFAN REGION, LORESTAN, Iran
Background: Phylogenetic background analyses have shown that E. coli strains fall into four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains be- long mainly to group B2; whereas most commensal strains belong to group...
Genotyping of E.coli Isolates from Children with Urinary Tract Infection by Pulse Field Gel Electrophoresis (Pfge)
Background: Urinary tract infection is one of the most im- portant diseases in children that delay of diagnosis and treat- ment may cause scar. E. coli because these infections in 75 % cases.Diarrheagenic E. coli have pathotypes but Pathotypes of E....
Homology of Multi Locus Sequence Types of H. Pylori Strains with Different Genotypes in a Single Host
Background: The purpose of this study was the investiga- tion of multiple sequence types of single isolates of H. pylori presenting diverse genotypes but identical RAPD patterns in a single host by MLST method.Methods: The gastric biopsy specimens were...
Identification of Multi-Drug Resistant Staphylococcus Aureus Isolates in Samples Obtained from Medical Foods, Kitchen Utensils and Food Handlers in a Hospital in Tehran
Background: Multi- drug resistant (MDR) S. aureus is one of the most important etiological factors in food-borne diseases in predisposed patients at medical centers. This study was carried out to determine the frequency of MDR- S. aureus isolates in...
IDENTIFYING OF CAUSATIVE AGENTS OF CUTANEOUS LEISHMANIASES BY AMPLIFYING CYT B GENE IN INDIGENOUS FOCI OF Iran
Background: In this study, simultaneously identifying of three causative agents species of CL is followed by targeting Cyt b gene in three regions of Turkmen Sahara, Ahvaz and Abarkouh.Methods: Smears of suspected patients infected with zoono- tic cutaneous...
Increasing Isolation Rate of Mott in South of Turkey
Background: Mycobacteria other than tuberculosis (MOTT) are environmental organisms capable of causing chronic dis- ease in humans and cause increasingly serious infections es- pecially in immunosuppressive patients. The genus Mycobacte- rium currently...
Intrafamilial Transmission of Helicobacter Pylori: Genotyping of Fecal Samples
Background: Helicobacter pylori (H. pylori) commonly acquired in early childhood. After more than 20 years of research, there is a little information about the details routes of this bacterium transmission. The aim of this study was to explore intrafamilial...
INVESTIGATING THE EFFECT OF COTRIMAXAZOL AS PROPHYLAXIS FOR PREVENTING URINARY TRACT INFECTION CAUSED BY KLEBSIELLA AFTER THE CESAREAN SECTION IN HOSPITALS OF SOUTH OF Iran
Background: Since one of the infections that might occur after the cesarean section is the urinary tract infections, this study aims at investigating the general prevalence of this kind of infection caused by Cotrimaxazol-resistant Klebsiella, as well...
Investigation of Antimicrobial Activity of Total Extraction and Fractions of Twigs of Gailonia Aucheri against Different Bacterial Strains and Candida Spp
Background: The aim of this research was to study of the antimicrobial activity of total extract and petroleum ether, chloroform, ethyl acetate, methanol and aqueous fractions of twigs of Gailonia aucheri.Methods: Aerial top branches of plant G. aucheri...
Investigation of Tigecycline Resistance among Metallo-Beta-Lactamase Producing Acinetobacter Baumannii Isolates in an Educational Hospital of Esfahan
Background: This study was designed to investigate the tigecycline resistance among metallo-beta-lactamase produc- ing Acinetobacter baumannii isolates in an educational hospital of Esfahan.Methods: In this cross-sectional study, 107 A. baumannii recovered...
In Vivo Bioactivity of Truncated Streptokinase Lacking 42 Amino Acids in C-Terminal Region
Background: In the present study, the bioactivity of the recombinant streptokinase (rSK) was evaluated in vivo.Methods: Streptokinase mutant gene, lacking 42 amino ac- ids, was cloned in pET32a. Expression of the rSK was con- firmed by western blotting...
Isolation and Characterization of Antibiotic Producer Actinomycetes Species from the Persian Gulf
Background: To evaluate the antimicrobial activity of acti- nomycetes isolated from the Persian Gulf, as untapped source for searching new natural antibiotics.Methods: Initially, water and sediment samples were collect- ed from 23 study sites in the...
Isolation and Characterization of Methane- Oxidizing Bacteria from Soil
Background: The main purpose of current study is isolation and characterization of the bacteria which are capable of me- thane consumptionas the sole source carbon and energy.Methods: Soil samples were collected from the oil contami- nated regions. The...
Isolation and Identification of Bacillus Subtilis from the Soil Saturated Salt Lake Qom and the Safety as Food Probiotics
Background: Health promoting microorganisms, probiotics, have been recently increasingly included in various food products and proposed for use as a food supplement or as a therapy for several infectious diseases. Probiotics based on Bacillus strains...
Isolation and Identification of Environmental Halophilic Bacteria Producing Industrially Important Enzymes from Salty Soils
Background: Industrially important enzymes are endowed with catalytic power to sustain microbial metabolic and phys- iological processes under salt conditions. The aim of the study was isolation and derivation of halophilic bacteria pro- ducing enzymes...
Mechanism of Antibacterial Action of Satureja Khuzistanica Essential Oil
Background: The essential oil of Satureja khuzistanica exhib- its broad-spectrum antimicrobial activity. The major compo- nent of S. khuzistanica essential oil (EO) is shown to be car- vacrol. We studied the mechanism of S. khuzistanica EO ac- tion on...
MICROBIAL ANALYSIS OF VEGETABLES IN Iran DURING PAST 5 YEARS
Background: We aimed the Meta analysis study on preva- lence of microbes transmitted by vegetables in Iran during 5 past years.Methods: In the current Meta-analysis study, by using key- words such as Iran, vegetable, microbial contamination, and using...
MICROBIOLOGY OF IranIAN PROBIOTIC WHITE CHEESE MADE WITH DIFFERENT FAT CONTENTS
Background: Iranian White cheeses were produced in three different fat content with Freeze-dried mixed mesophilic- thermophilic culture (blend of Lactococcus lactis subsp. Lactis strain 227 and Lc. lactis subsp. cremoris strains 223) and probiotic strains(Lb....
MOLECULAR CHARACTERISTICS OF HEPATITIS B VIRUS ISOLATED FROM INTRAVENOUS DRUG USERS IN TEHRAN-Iran, 2013
Background: We investigated the possible genotypes in HBV-infected IVDU in Tehran.Methods: This study was conducted on 229 intravenous drug users who referred to three Drop- in-Centers and Loghman hospital in Tehran. Their serum samples were test- ed...
Molecular Fingerprinting as a Tool for Surveillance Study of Foodborne Infections in Hospital Settings
Background: Hospital acquired infections are still a major healthcare problem in the world. Hospitals have been identi- fied as high food safety risk institutions because they serve potentially hazardous foods to vulnerable people. The aim of this study...
Molecular Identification of Esbl Genes Bla -Shv, Bla-Veb, and Bla-Per, in Acinetobacter Baumannii Isolated from Patients Admitted in a University Hospital in Isfahan
Background: Acinetobacter baumannii has become one of the most important pathogens responsible for healthcare-associated infections and particularly affects pa- tients admitted to intensive care units (ICUs). Prevalence of class extended-spectrum -lactamases...
MOLECULAR STUDY OF BRUCELLOSIS IN ENDEMIC REGIONS OF Iran BASED ON NOVEL URS-PCR METHOD
Background: Brucellosis is an infectious disease in human and animals. Bacteriologic-culture-based methods used for Brucella diagnosis are hard and time consuming. The aim of this study was to introduce a novel molecular method for detection of two prevalent...
MOLECULLAR TYPING OF ESBL PRODUCING UROPATHOGENIC ESCHERICHIA COLI IN WEST OF Iran
Background: Urinary tract infection (UTI) is one of the most common bacterial infections in the world. Cephalospor- in'sare commonly used for treatment of infections by Esche- richia coli in UTI. However, resistance to these antibiotics is increasing...
Nested-Pcr as Gene-Mapping Tool to Recognize Length of Integron Class I among P.aeruginosa
Background: Integron as mobile elements could be help to induce resistance among species of bacteria. Three class of Integron is known and among them Integron class I by high frequency among P.aeruginosa play mainly role to resistant to antibiotics....
Nosocomial Infections and Related Factors in Southern Khorasan Hospitals
Background: This study aimed to determine the prevalence of nosocomial infections, the responsible microorganisms, and to identify the patients at high risk in hospitals with more than 100 beds in south Khorasan Province.Methods: In three hospital with...
Occurrence of Enterotoxin Gene Profile of Staphylococcus Aureus Isolated from Raw Milk of Cattle, Sheep and Goat and Pasteurized Milk Marketed in Shahrekord
Background: Nowadays, milk has been considered as one of the most important supplies to provide nutritional require- ments of human. However, milk and dairy products could be a source of contamination by different foodborne pathogens such as Staphylococcus...
Occurrence of Integrons and Antimicrobial Resistance Genes among Clinical Isolates of Enterobacter Spp. from Hospitals of Tehran
Background: The objectives of this study were to determine the prevalence of three classes of integrons in isolated Entero- bacter spp. and to find out the association between the pres- ence of integrons and antibiotic resistance.Methods: A total of...
Optimization of Benzene Biodegradation Using Streptomyces Isolated from Tabriz Refinery
Background: Streptomyces bacteria isolated from soil of Ta- briz Refinery was used for biodegradation of benzene. Then kinetic modeling of biodegradation and optimization of envi- ronmental conditions and pH were performed.Methods: After isolation and...
Optimization of Environmental Conditions of Azo B-Naphtol Orange Dye Decolorization by Halophilic Bacteria A3
Background: The most common synthetic dyes which are used widely in textile, pharmaceutical and food industries are Azo dyes and because of Diazo links are considered as im- portant biologically contaminants. Normally these dyes are resistant against...
Optimization of Methods for Isolating Environmental Mycobacteria from Water by the Parallel Application of 3 Isolation Methods
Background: Cultivation of mycobacteria requires long-term incubation in rich media and inactivation of rapidly growing microorganisms whose growth impedes observation of my- cobacterial colonies.Methods: 38 samples were collected from surface waters...
Optimization of Real Time Pcr for Detection of Brucell Abortus
Background: Brucellosis is a zoonotic disease transferred from infected animal to human. Six classic species involved B. abortus, B. melitensis, B. suis, B.canis, B.neotoma and B. ovis. In recent years four new species consist of B.ceti, B. maris, B....
Optimization of the Iron Bioleaching Parameters in Leptospirillum Ferriphilum UTMC 2299, Isolated from Gol-E-Gohar Iron Ore Mine
Background: In this study, we evaluated some physical and chemical parameters to determine the optimum conditions for Biooxidizing of ferrus iron to the ferric form by Lepto- spirillum ferriphilum UTMC 2299.Methods: Initially, the bacteria was cultured...
Over-Production of Epidermal Growth Factor (Egf) in E. Coli
Background: Epidermal Growth Factor (EGF) is a mono- mer polypeptide, which is produce from different tissue in human. It has many applications in medicine and Pharma- ceutical Science. The main source of EGF is Parotid gland. Production of EGF from...
Pathotypic Comparison of Urinary and Fecal Escherichiacoli Isolated from Children with Urinary Tract Infection
Background: Uropathogenic Escherichiacoli (UPEC) is a causative agent in most urinary tract infections (UTIs), ex- pressing a multitude of virulence factors. The aim of this study was to investigate the phylogenetic background and the presence of the...
Potential Risk of Acute Gastrointestinal Illness (Agi) in Young Children Associated with Swimming Pools Due to Pseudomonas Aeruginosa
Background: To assess if these swimming pools were a health risk to young children (age 7 to 11), thirteen public swimming pools, were examined and the exposed children were monitored by completing a questionnaire. An adequate control group was chosen...
Prevalence and Association of Helicobacter Pylori Infection with Gastrointestinal Disease
Background: Helicobacter pylori is one of the most infections in worldwide, it can colonize the gastric mucosa for years. The effect of Helicobacter pylori to cause gastrointestinal dis- eases is proved and also it can be associated with an increased...
Prevalence of Bacterial Contamination in Waterlines of Hamadan Dentistry School Units and Drinking Water Supply of Local Area
Background: The purpose of this study was to evaluate the bacterial contamination and biofilms formation of waterlines of dentistry units at dental faculty of in Hamadan University of Medical Sciences and drinking water sources contamina- tion of Hamadan...
PREVALENCE OF BLANDM, BLAPER, BLAVEB, BLAIMP AND BLAVIM GENES AMONG ACINETOBACTER BAUMANNII ISOLATED FROM TWO HOSPITALS OF TEHRAN, Iran
Background: The aim of this study was to determine the frequency of blaNDM, blaPER, bla VEB, blaIMP and bla- VIM type genes among A. baumannii isolates from hospital- ized patients in Milad and Loghman Hakim hospitals, Teh- ran-Iran from 2012 to 2013.Methods:...
Prevalence of Coagulase Positive Pathogenic Staphylococcus Aureus in Mayonnaise Sauce
Background: This study was done to determine the preva- lence of coagulase positive pathogenic Staphylococcus aureus strains isolated from mayonnaise sauce and drug sensitivity pattern.Methods: In this experimental study, we used of 120 packets (sachet)...
Prevalence of Ctx-3 (Ctx-M 3,15,22) Family Gene in Various E.coli and Klebsiella Pneumoniae Clinical Specimes in Tabriz
Background: Beta-lactam antibiotics are the most frequently prescribed antibiotics. The emergence of resistance to these antibiotics among gram-negative bacilles has limited their efficiency. This study was done to determine the frequency of producing...
Prevalence of Fluoroquinolone-Resistance among Extended-Spectrum B Lactamase Producing Klebsiella Isolates from Clinical Specimens
Background: Klebsiella pneumonia is one of the most common causes of nosocomial infections causinghigh rate of mortality. However, most isolates of Klebsiella pneumoniae are multidrug resistant and increasing the emergence of ESBL-mediated resistance...
Prevalence of Listeria Species in Raw Milk in East-Azerbaijan Province
Background: The genus Listeria consists of six species amongst L. monocytogenes and L. ivanovii are pathogenic to hu- mans and cause listeriosis. Listeriosis may be transmitted through direct contact with infected animals or by consump- tion of contaminated...
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