Genetics

Professional journal providing news and analysis on field of genetics. Features special reports such as policy reviews and awards.

Articles from Vol. 191, No. 3, July

Age-Specific Variation in Immune Response in Drosophila Melanogaster Has a Genetic Basis
ABSTRACT Immunosenescence, the age-related decline in immune system function, is a general hallmark of aging. While much is known about the cellular and physiological changes that accompany immunosenescence, we know little about the genetic influences...
A Hyperactive Transposase of the Maize Transposable Element Activator (Ac)
ABSTRACT Activator/Dissociation (Ac/Ds) transposable elements from maize are widely used as insertional mutagenesis and gene isolation tools in plants and more recently also in medaka and zebrafish. They are particularly valuable for plant species that...
A Regulatory Module Controlling Pharyngeal Development and Function in Caenorhabditis Elegans
ABSTRACT In Caenorhabditis elegans, the differentiation and morphogenesis of the foregut are controlled by several transcriptional regulators and cell signaling events, and by PHA-1, an essential cytoplasmic protein of unknown function. Previously we...
A Role for siRNA in X-Chromosome Dosage Compensation in Drosophila Melanogaster
ABSTRACT Sex-chromosome dosage compensation requires selective identification of X chromatin. How this occurs is not fully understood. We show that small interfering RNA (siRNA) mutations enhance the lethality of Drosophila males deficient in X recognition...
Biomechanical Profiling of Caenorhabditis Elegans Motility
ABSTRACT Caenorhabditis elegans locomotion is a stereotyped behavior that is ideal for genetic analysis. We integrated video microscopy, image analysis algorithms, and fluid mechanics principles to describe the C. elegans swim gait. Quantification of...
Chaperoning the Chaperone: A Role for the Co-Chaperone Cpr7 in Modulating Hsp90 Function in Saccharomyces Cerevisiae
ABSTRACT Heat-shock protein 90 (Hsp90) of Saccharomyces cerevisiae is an abundant essential eukaryotic molecular chaperone involved in the activation and stabilization of client proteins, including several transcription factors and oncogenic kinases....
Chromatin Modulation at the FLO11 Promoter of Saccharomyces Cerevisiae by HDAC and Swi/Snf Complexes
ABSTRACT Cell adhesion and biofilm formation are critical processes in the pathogenicity of fungi and are mediated through a family of adhesin proteins conserved throughout yeasts and fungi. In Saccharomyces cerevisiae, Flo11 is the main adhesin involved...
Cytoplasmic and Genomic Effects on Meiotic Pairing in Brassica Hybrids and Allotetraploids from Pair Crosses of Three Cultivated Diploids
ABSTRACT Interspecific hybridization and allopolyploidization contribute to the origin of many important crops. Synthetic Brassica is a widely used model for the study of genetic recombination and "fixed heterosis" in allopolyploids. To investigate the...
Disentangling the Roles of History and Local Selection in Shaping Clinal Variation of Allele Frequencies and Gene Expression in Norway Spruce (Picea Abies)
ABSTRACT Understanding the genetic basis of local adaptation is challenging due to the subtle balance among conflicting evolutionary forces that are involved in its establishment and maintenance. One system with which to tease apart these difficulties...
Evidence for a Natural Allelic Series at the Maize Domestication Locus Teosinte Branched1
ABSTRACT Despite numerous quantitative trait loci and association mapping studies, our understanding of the extent to which natural allelic series contribute to the variation for complex traits is limited. In this study, we investigate the occurrence...
Fine-Scale Maps of Recombination Rates and Hotspots in the Mouse Genome
ABSTRACT Recombination events are not uniformly distributed and often cluster in narrow regions known as recombination hotspots. Several studies using different approaches have dramatically advanced our understanding of recombination hotspot regulation....
Genetic Evidence Links the ASTRA Protein Chaperone Component Tti2 to the SAGA Transcription Factor Tra1
ABSTRACT Tra1 is a 3744-residue component of the Saccharomyces cerevisiae SAGA, NuA4, and ASTRA complexes. Tra1 contains essential C-terminal PI3K and FATC domains, but unlike other PIKK (phosphoinositide three-kinase-related kinase) family members,...
Genetics and Evolution of Hybrid Male Sterility in House Mice
ABSTRACT Comparative genetic mapping provides insights into the evolution of the reproductive barriers that separate closely related species. This approach has been used to document the accumulation of reproductive incompatibilities over time, but has...
Improved Models for Transcription Factor Binding Site Identification Using Nonindependent Interactions
ABSTRACT Identifying transcription factor (TF) binding sites is essential for understanding regulatory networks. The specificity of most TFs is currently modeled using position weight matrices (PWMs) that assume the positions within a binding site contribute...
Issue Highlights
Increasing association mapping power and resolution in mouse genetic studies through the use of meta-analysis for structured populations, pp. 959-967Nicholas A. Furlotte, Eun Yong Kang, Atila Van Nas, Charles R. Farber, Aldons J. Lusis, and Eleazar EskinBecause...
Megabase-Scale Inversion Polymorphism in the Wild Ancestor of Maize
ABSTRACT Chromosomal inversions are thought to play a special role in local adaptation, through dramatic suppression of recombination, which favors the maintenance of locally adapted alleles. However, relatively few inversions have been characterized...
Multiple Barriers to Nonhomologous DNA End Joining during Meiosis in Drosophila
ABSTRACT Repair of meiotic double-strand breaks (DSBs) uses the homolog and recombination to yield crossovers while alternative pathways such as nonhomologous end joining (NHEJ) are suppressed. Our results indicate that NHEJ is blocked at two steps of...
Notch and the Awesome Power of Genetics
ABSTRACT Notch is a receptor that mediates cell-cell interactions in animal development, and aberrations in Notch signal transduction can cause cancer and other human diseases. Here, I describe the major advances in the Notch field from the identification...
Properties and Power of the Drosophila Synthetic Population Resource for the Routine Dissection of Complex Traits
ABSTRACT The Drosophila Synthetic Population Resource (DSPR) is a newly developed multifounder advanced intercross panel consisting of >1600 recombinant inbred lines (RILs) designed for the genetic dissection of complex traits. Here, we describe the...
Remarkably Simple Sequence Requirement of the M-Factor Pheromone of Schizosaccharomyces Pombe
ABSTRACT +he mating reaction is triggered by specific pheromones in a wide variety of organisms. Small peptides are used as mating pheromones in yeasts and fungi. In +he fission yeast Schizosaccharomyces pombe, M-factor is a C terminally farnesylated...
Ribosomal DNA Organization before and after Magnification in Drosophila Melanogaster
ABSTRACT In all eukaryotes, the ribosomal RNA genes are stably inherited redundant elements. In Drosophila melanogaster, the presence of a Ybb2 chromosome in males, or the maternal presence of the Ribosomal exchange (Rex) element, induces magnification:...
RNA Degradation in Saccharomyces Cerevisae
ABSTRACT All RNA species in yeast cells are subject to turnover. Work over the past 20 years has defined degradation mechanisms for messenger RNAs, transfer RNAs, ribosomal RNAs, and noncoding RNAs. In addition, numerous quality control mechanisms that...
Temporal Variation in Selection Accelerates Mutational Decay by Muller's Ratchet
ABSTRACT Asexual species accumulate deleterious mutations through an irreversible process known as Muller's ratchet. Attempts to quantify the rate of the ratchet have ignored the role of temporal environmental heterogeneity even though it is common in...