Relationship between the Prevalence of Campylobacter Spp. in Caspian Sea's Water and Various Types of Fishes Using Pcr Technique

By Poplawski, Waldemar; Ghane, Masoud | Iranian Journal of Public Health, January 1, 2014 | Go to article overview

Relationship between the Prevalence of Campylobacter Spp. in Caspian Sea's Water and Various Types of Fishes Using Pcr Technique


Poplawski, Waldemar, Ghane, Masoud, Iranian Journal of Public Health


Background: The major purpose of this study was isolation, identification and characterization of Campylobacter spp. from Caspian Sea's water in north of Iran and various types of fishes obtained from this sea.Campylobacteris one of the most common causes of diarrhea world wide. According to world health organization estimated 2-35% of diarrhea induced by Campylobacter. So that, annually 400-500 million peoples in the word are infected with these bacteria. Disease in human be- ing occurs through consumption of contaminated water and animal products viz., meat and milk.

Methods: In total, 70 water samples and 165 samples were obtained from south coastal Caspian Sea and various types of fishes obtained from this area in one year.The isolates were characterized by using standard Campylobacter phenotypic identification tests recommended by Atabay and Corry. At the end, the PCR method was carried out in order to confirm the phenotyping results.

Results: After conducting of the Phenotyping methods and their confirmation with molecular technique, three strain of Campylobacter jejuni were identified. …

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