Histological Technique: A Guide for Use in a Laboratory Course in Histology

By B. F. Kingsbury; O. A. Johannsen | Go to book overview

MOUNTING
149. Whether stained or unstained, prepared for microscopical examination by isolation or sectioning, and especially if it is desired to keep the preparation, it is necessary to mount it in some way, i.e., so arrange it upon some suitable support (glass slide) and in some suitable mounting medium that it may be satisfactorily studied with the microscope.Mounting may be:
I. I. Temporary, or
II. II. Permanent, -- as
A. A. Dry, or in air,
B. B. In a medium miscible with water, or
C. C. In a resinous medium, in which case it is necessary first to remove all water by either (a) drying -- desiccation, or (b) a series of displacements, i.e., (1) Removing the water with strong alcohol -- dehydration; (2) Removing the alcohol with clearer -- clearing; (3) Replacing the clearer with balsam or other resinous mounting medium.
150. Temporary Mounting. Illustrations may be found in the examination of blood corpuscles and living ciliated cells (§ 39). Temporary examination of tissues is quite simple, though important, and for this it is only necessary to place the teased tissue or section on the slide in a drop of the fluid in which it is at the time, normal salt solution, dissociator, or alcohol, and cover. The examination of preparations intended for permanent mounts during the staining or before mounting will often serve to detect faulty treatment at a time when it may be remedied without great expenditure of time, or to discard the specimen as worthless.151. Indifferent Media. These are of much wider usefulness, and are numerous. There may be mentioned:
1. Physiological salt solution. Distilled water, 100 cc.; common salt, 0.7 gram. As applied to vertebrates, it is customary to take, for amphibia, 0.6 gram; reptiles and birds, 0.75 gram; mammals, 0.9 gram, of the sodium chlorid.
2. Ringer's solution (modified). Sodium chlorid, 0.80 gram; calcium chlorid, 0.02 gram; potassium chlorid, 0.02 gram; sodium bicarbonate, 0.02 gram; distilled water, 100 cc.

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Histological Technique: A Guide for Use in a Laboratory Course in Histology
Table of contents

Table of contents

  • Title Page i
  • Preface iii
  • Contents v
  • Introduction vii
  • Fixation 1
  • Isolation 12
  • Imbedding Methods 17
  • Staining 37
  • Mounting 56
  • Special Methods 70
  • Special Methods for Various Animal Forms 107
  • Appendix - Suggestions to the Beginner on a Procedure For Fixing, Sectioning, and Staining Histological Specimens -- Paraffin Method 131
  • References 133
  • Index 137
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