Eggert used these properties for the qualitative detection of carbon monoxide poisoning. If a drop of blood poisoned with carbon monoxide is photographed by infrared on a glass slide next to a drop of healthy blood, it will appear transparent while the healthy blood will appear dark. Eggert published absorption curves of the blood used in his experiments, and they substantiated the photographic findings. His work was extended further by Merkelbaeh71 who confirmed the strong absorption band of oxyhemoglobin with its peak just beyond 9,000 A and the absence of such absorption in the case of carbonylhemoglobin.
The study was resumed by Horecker and his collaborators3, 44, 45 who worked out a photoelectric spectrophotometric method for determining methemoglobin, carbonylhemoglobin, and total hemoglobin in blood, using the infrared absorption charaeteristics. They have published the spectral absorption curves of various hemoglobin derivatives out to 10,000 A.
See also references at end of Chapter XI.
1. ADAMS E. Q., "The Penetration of Radiation," Phys. Rev., 1930, 36, 1020.
2. ALDRICH L. B., "Supplementary Notes bon Body Radiation," Smithsonian Inst. Misc. Collections, 1932, 85, no. 11.
3. ANDREWS H. I., and HORECKER B. L., "A Simplified Photoelectric Colorimeter for Blood Analysis," Rev. Sci. Instruments, 1945, 18, 148-52.
4. ANGUS T. C., and TAYLOR H. J., "Measurements of Transmission of Radiation through a Non-Homogeneous Medium, viz., the Horny Layer of the Human Skin," Proc. Roy. Soc., B, 1931-32, 109, 309-18.
5. BACHEM A., and REED C. I., "The Penetration of Light through Human Skin," Am. J. Physiol., 1931, 97, 86-91.
6. BALDERRY F. C., and EWALD E., "Life Energy in Therapeutics," Am. Rev. Tuberc., 1924, 8, 501-18.
7. BARKER N. W., and JULINS L. A., "Demonstration of Superficial Veins by Infra-Red Photography," Proc. Staff Meetings Mayo Clinic, 1934, 9 (ser. V), 68-70.
8. BAZETT H. C., and McGLONE B., "Temperature Gradients in the Tissues in Man," Am. J. Physiol., 1927, 82, 415-51.
9. BLAIR D. M., and DAVIES F., "Infra-Red Plates in Neurohistological Illustration," Lancet, 1933, 225, 801-02.