Cytological Technique: The Principles Underlying Routine Methods

By John R. Baker | Go to book overview

overstain when dissolved in absolute alcohol. Its colour and transparency make orange G a good counterstain for blue and black basic dyes and dyelakes.

A number of acid disazo dyes (e.g., trypan blue and red, vital red) and the trisazo chlorazol black are used in vital studies of the reticulo-endothelial system. When solutions of these dyes are injected subcutaneously or into the blood stream, colloidal aggregates of them are taken up by phagocytic cells and held so firmly that they can be clearly seen when tissues are subsequently fixed, sectioned, and stained in contrasting colours.

Sudan III and IV are fat-soluble disazo compounds. They are not dyes (see p. 139).


NITRO DYES

In these the chromophore is the nitro-group, --NO2. Picric acid is an example. This is the only substance that is used as a fixative (p. 58), as a dye, and as a differentiator (p. 151). It was in the past used a good deal for contrast with carmine, and Altmum used it for replacing acid fuchsine in cytoplasm while leaving the latter dye in mitochondria. For this purpose another, more complex, nitro dye is preferable, namely aurantia (see p. 188). This is not a free acid, but an ammonium compound.


CONCLUSION

Very few dyes have been mentioned in this chapter, In comparison with the number that have been used in one way or another in micro-technique. It is hoped that enough has been said to make it possible for the reader to understand something of the structure and mode of action of dyes in general and to use any dye intelligently. In the textile industry vast numbers of dyes are used to try to satisfy every whim of aesthetic appreciation, but in cytology a vast array is unnecessary. The cytologist who can show

-171-

Notes for this page

Add a new note
If you are trying to select text to create highlights or citations, remember that you must now click or tap on the first word, and then click or tap on the last word.
One moment ...
Default project is now your active project.
Project items
Notes
Cite this page

Cited page

Style
Citations are available only to our active members.
Buy instant access to cite pages or passages in MLA 8, MLA 7, APA and Chicago citation styles.

(Einhorn, 1992, p. 25)

(Einhorn 25)

(Einhorn 25)

1. Lois J. Einhorn, Abraham Lincoln, the Orator: Penetrating the Lincoln Legend (Westport, CT: Greenwood Press, 1992), 25, http://www.questia.com/read/27419298.

Note: primary sources have slightly different requirements for citation. Please see these guidelines for more information.

Cited page

Bookmark this page
Cytological Technique: The Principles Underlying Routine Methods
Table of contents

Table of contents

  • Title Page iii
  • Preface to the Second Edition v
  • Preface vi
  • Contents vii
  • Chapter I - Introduction 1
  • Chapter III - Simple Fixatives 21
  • Chapter IV - Fixing Mixtures 42
  • Chapter V - Microtomy 104
  • Conclusion 171
  • Chapter VIII - Methods for Chromosomes, Mitochondria and the Golgi Element 172
  • List of References 198
  • Index 205
Settings

Settings

Typeface
Text size Smaller Larger Reset View mode
Search within

Search within this book

Look up

Look up a word

  • Dictionary
  • Thesaurus
Please submit a word or phrase above.
Print this page

Print this page

Why can't I print more than one page at a time?

Help
Full screen
Items saved from this book
  • Bookmarks
  • Highlights & Notes
  • Citations
/ 214

matching results for page

    Questia reader help

    How to highlight and cite specific passages

    1. Click or tap the first word you want to select.
    2. Click or tap the last word you want to select, and you’ll see everything in between get selected.
    3. You’ll then get a menu of options like creating a highlight or a citation from that passage of text.

    OK, got it!

    Cited passage

    Style
    Citations are available only to our active members.
    Buy instant access to cite pages or passages in MLA 8, MLA 7, APA and Chicago citation styles.

    "Portraying himself as an honest, ordinary person helped Lincoln identify with his audiences." (Einhorn, 1992, p. 25).

    "Portraying himself as an honest, ordinary person helped Lincoln identify with his audiences." (Einhorn 25)

    "Portraying himself as an honest, ordinary person helped Lincoln identify with his audiences." (Einhorn 25)

    "Portraying himself as an honest, ordinary person helped Lincoln identify with his audiences."1

    1. Lois J. Einhorn, Abraham Lincoln, the Orator: Penetrating the Lincoln Legend (Westport, CT: Greenwood Press, 1992), 25, http://www.questia.com/read/27419298.

    Cited passage

    Thanks for trying Questia!

    Please continue trying out our research tools, but please note, full functionality is available only to our active members.

    Your work will be lost once you leave this Web page.

    Buy instant access to save your work.

    Already a member? Log in now.

    Search by... Author
    Show... All Results Primary Sources Peer-reviewed

    Oops!

    An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.