Mitochondria and Other Cytoplasmic Inclusions

By The Company Of Biologists | Go to book overview

EXPLANATION OF PLATES
The following abbreviations are used throughout to indicate the objectives with which the photographs were taken: (1) C.T.S.P.C. = Cooke, Troughton and Simms 1.8 mm. phase- contrast oil-immersion objective. (2) N.Z.P.C. = Nedoptifa Zeist 2 mm. oil-immersion phase-contrast objective. (3) C.T.S.A. = Cooke, Troughton and Simms 2.2 mm. oil- immersion apochromatic objective. The scale on the photographs in all cases represents a distance of 10μ. When there is more than one photograph of the same cell their magnification is always the same.
PLATE 1
Fig. 1. Living unstained snail amoebocyte containing considerable number of lipid granules, together with one large neutral-red inclusion (A). At the upper pole of the latter there are two small neutral-red inclusions and three more at its lower pole, one of which is labelled B. CTSPC.
Fig. 2. The same cell as in fig. 1 after fixation in 1% osmium tetroxide and post-chroming in saturated potassium dichromate. The lipid granules are well preserved. The large neutral-red inclusion is paler as a result of partial loss of its solid content, while the small neutral-red inclusions adjacent to its upper and lower poles are moderately well preserved. The lowest of the small neutral-red spheres (B in fig. 1) has been destroyed. Mitochondria can still be distinguished. CTSPC.
Fig. 3. The same cell as in fig. 2 after treatment with sudan black. The lipid granules are intensely blackened. The large neutral-red sphere is unstained, whereas the two smaller ones at its upper and lower poles are coloured very faintly. CTSA.
Fig. 4. Living unstained snail amoebocyte, containing a variety of neutral-red inclusions, some of which indent the nucleus. There are also rod-shaped mitochondria and some lipid granules. CTSPC.
Fig. 5. The same cell as in fig. 4 after it has been stained supravitally with 0.00005% neutral red. The small inclusions, which lie on the periphery of the large sphere, have enlarged, but otherwise there has been little morphological change. CTSPC.
Fig. 6. The same cell as in fig. 5, photographed to show the neutral-red staining. All of the neutral-red inclusions have been coloured but with varying intensity. CTSA.
Fig. 7. Living unstained snail amoebocyte containing a fairly large and apparently homogeneous neutral-red sphere (A). There are also some smaller neutral-red inclusions and lipid granules. CTSPC.
Fig. 8. The same cell as in fig. 7 after supravital staining with 0.00005% neutral red. The large sphere (A) is no longer homogeneous, and consists of dark and lightly staining regions. CTSA.

PLATE 2
Fig. 9. Living unstained snail amoebocyte, containing a moderate-sized neutral-red sphere to the left of, and indenting, the nucleus, together with small neutral-red inclusions at its lower pole and lipid granules at its upper pole. CTSPC.
Fig. 10. The same cell as in fig. 9 after supravital staining with 0.00005% neutral red. The neutral-red inclusions have stained, and in addition a new coloured sphere (A) has appeared. The lipid granules are uncoloured. CTSA.
Fig. 11. A living unstained snail amoebocyte containing a fairly dense neutral-red sphere lying in an indentation of the nucleus. CTSPC.
Fig. 12. The same cell as in fig. 11 after 10 min. fixation in formal calcium. The neutral- red sphere has become pale as a result of loss of its solid contents and decrease of its refractive index. CTSPC.
Fig. 13. Living unstained snail amoebocyte containing two neutral-red spheres (A and B) and some lipid granules. CTSPC.
Fig. 14. The same cell as in fig. 13 after fixation in 1 % osmium tetroxide, post-chroming with saturated potassium dichromate, and treatment with sudan black. The neutral-red inclusions (A and B) have been blackened extremely faintly, although re-examination with phase-contrast microscopy showed that their solid content had been quite well preserved. The lipid granules have been blackened intensely. CTSA.
Fig. 15. Living unstained snail amoebocyte containing a fairly dense neutral-red sphere above the nucleus. CTSPC.

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